TY - JOUR
T1 - The polymerase chain reaction in the study of mitochondrial genetics
AU - Kapsa, Robert
AU - Siregar, Nurjati
AU - Quigley, Anita
AU - Ojaimi, Joseline
AU - Katsabanis, Sophie
AU - Sue, Carolyn
AU - Byrne, Edward
N1 - Funding Information:
This work was supported by National Health and Medical Research Council project grants 940206 and 960716. J.O. is a recipient of Australian Post-graduate Research Award. The authors would like also to thank Dr Hamit Ayberk and Dr David Kaufman for their valuable advice with in situ PCR and histological analyses respectively.
PY - 1997/12/17
Y1 - 1997/12/17
N2 - Since its development in the late 1980's, the polymerase chain reaction (PCR) has revolutionised molecular genetic studies. It has provided direct access to genetic material in quantities sufficient for meaningful analyses to be performed. Adaptations to the basic technique have resulted in a wide range of applications from basic gene amplification to the estimation of DNA species quantities within cells. The study of human mitochondrial genetics is but one of the many disciplines to benefit from the rapid ascension of PCR based technology. In this communication we outline several uses of the PCR technique in the detection, quantification and characterisation of human mitochondrial genetic defects. The data presented in this communication highlight the versatility and applicability of PCR not only to mitochondrial research but to other disciplines of medical research.
AB - Since its development in the late 1980's, the polymerase chain reaction (PCR) has revolutionised molecular genetic studies. It has provided direct access to genetic material in quantities sufficient for meaningful analyses to be performed. Adaptations to the basic technique have resulted in a wide range of applications from basic gene amplification to the estimation of DNA species quantities within cells. The study of human mitochondrial genetics is but one of the many disciplines to benefit from the rapid ascension of PCR based technology. In this communication we outline several uses of the PCR technique in the detection, quantification and characterisation of human mitochondrial genetic defects. The data presented in this communication highlight the versatility and applicability of PCR not only to mitochondrial research but to other disciplines of medical research.
KW - Human mitochondrial genetics
KW - In situ PCR and long PCR
KW - Polymerase chain reaction (PCR)
UR - http://www.scopus.com/inward/record.url?scp=0031409609&partnerID=8YFLogxK
U2 - 10.1016/S0165-022X(97)00044-4
DO - 10.1016/S0165-022X(97)00044-4
M3 - Article
C2 - 9507371
AN - SCOPUS:0031409609
SN - 1874-3919
VL - 36
SP - 31
EP - 50
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
IS - 1
ER -