The polymerase chain reaction in the study of mitochondrial genetics

Robert Kapsa, Nurjati Siregar, Anita Quigley, Joseline Ojaimi, Sophie Katsabanis, Carolyn Sue, Edward Byrne

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Since its development in the late 1980's, the polymerase chain reaction (PCR) has revolutionised molecular genetic studies. It has provided direct access to genetic material in quantities sufficient for meaningful analyses to be performed. Adaptations to the basic technique have resulted in a wide range of applications from basic gene amplification to the estimation of DNA species quantities within cells. The study of human mitochondrial genetics is but one of the many disciplines to benefit from the rapid ascension of PCR based technology. In this communication we outline several uses of the PCR technique in the detection, quantification and characterisation of human mitochondrial genetic defects. The data presented in this communication highlight the versatility and applicability of PCR not only to mitochondrial research but to other disciplines of medical research.

Original languageEnglish
Pages (from-to)31-50
Number of pages20
JournalJournal of Biochemical and Biophysical Methods
Issue number1
Publication statusPublished - 17 Dec 1997


  • Human mitochondrial genetics
  • In situ PCR and long PCR
  • Polymerase chain reaction (PCR)


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