TY - GEN
T1 - The optimization of nanofiber chitin, and chitosan concentrations as encapsulation materials of Lactobacillus casei probiotic
AU - Djaenudin,
AU - Saepudin, Endang
AU - Nasir, Muhamad
N1 - Funding Information:
The authors were grateful to LIPI for supporting financial through by research program and ELSA for analytical instruments.
Publisher Copyright:
© 2022 Author(s).
PY - 2022/12/5
Y1 - 2022/12/5
N2 - Chitosan and nanofiber chitin could safeguard L. casei from a stomach environment. The objective of this research was to determine the effectivity of the encapsulation formulation on the L. casei survivability within an SGF. The beads forming was done by extrusion. Encapsulation materials were varied using 1% alginate, nanofiber chitin (0.2%, 0.6%, 1%, and 2%) and chitosan (0.2%, 0.6%, 1%, and 2%) on the flow rate 1 mL/min and the voltage 0 kV. The resulting beads were immersed for 1, 60, and 120 minutes at 37°C within SGF (pH of 1.2 and 3). The higher chitosan or nanofiber chitin concentration, the higher survivability of L. casei within SGF. The composition of chitosan (2%) and nanofiber chitin (2%) as the encapsulation materials showed the highest viability of 8.08 log CFU/g (survivability of 70.31%) and 9.40 log CFU/g (survivability of 81.78%) within SGF pH 1.2 and 3 for 120 min, respectively.
AB - Chitosan and nanofiber chitin could safeguard L. casei from a stomach environment. The objective of this research was to determine the effectivity of the encapsulation formulation on the L. casei survivability within an SGF. The beads forming was done by extrusion. Encapsulation materials were varied using 1% alginate, nanofiber chitin (0.2%, 0.6%, 1%, and 2%) and chitosan (0.2%, 0.6%, 1%, and 2%) on the flow rate 1 mL/min and the voltage 0 kV. The resulting beads were immersed for 1, 60, and 120 minutes at 37°C within SGF (pH of 1.2 and 3). The higher chitosan or nanofiber chitin concentration, the higher survivability of L. casei within SGF. The composition of chitosan (2%) and nanofiber chitin (2%) as the encapsulation materials showed the highest viability of 8.08 log CFU/g (survivability of 70.31%) and 9.40 log CFU/g (survivability of 81.78%) within SGF pH 1.2 and 3 for 120 min, respectively.
KW - chitosan
KW - L. casei
KW - nano chitin
UR - http://www.scopus.com/inward/record.url?scp=85144534829&partnerID=8YFLogxK
U2 - 10.1063/5.0111565
DO - 10.1063/5.0111565
M3 - Conference contribution
AN - SCOPUS:85144534829
T3 - AIP Conference Proceedings
BT - Proceedings of the 7th International Symposium on Applied Chemistry 2021
A2 - Al Muttaqii, Muhammad
A2 - Kurniawan, Kiki
A2 - Hanafi, Muhammad
A2 - Randy, Ahmad
PB - American Institute of Physics Inc.
T2 - 7th International Symposium on Applied Chemistry 2021, ISAC 2021
Y2 - 28 September 2021 through 30 September 2021
ER -