The optimization of nanofiber chitin, and chitosan concentrations as encapsulation materials of Lactobacillus casei probiotic

Djaenudin, Endang Saepudin, Muhamad Nasir

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Chitosan and nanofiber chitin could safeguard L. casei from a stomach environment. The objective of this research was to determine the effectivity of the encapsulation formulation on the L. casei survivability within an SGF. The beads forming was done by extrusion. Encapsulation materials were varied using 1% alginate, nanofiber chitin (0.2%, 0.6%, 1%, and 2%) and chitosan (0.2%, 0.6%, 1%, and 2%) on the flow rate 1 mL/min and the voltage 0 kV. The resulting beads were immersed for 1, 60, and 120 minutes at 37°C within SGF (pH of 1.2 and 3). The higher chitosan or nanofiber chitin concentration, the higher survivability of L. casei within SGF. The composition of chitosan (2%) and nanofiber chitin (2%) as the encapsulation materials showed the highest viability of 8.08 log CFU/g (survivability of 70.31%) and 9.40 log CFU/g (survivability of 81.78%) within SGF pH 1.2 and 3 for 120 min, respectively.

Original languageEnglish
Title of host publicationProceedings of the 7th International Symposium on Applied Chemistry 2021
EditorsMuhammad Al Muttaqii, Kiki Kurniawan, Muhammad Hanafi, Ahmad Randy
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735442658
DOIs
Publication statusPublished - 5 Dec 2022
Event7th International Symposium on Applied Chemistry 2021, ISAC 2021 - Virtual, Online
Duration: 28 Sept 202130 Sept 2021

Publication series

NameAIP Conference Proceedings
Volume2493
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference7th International Symposium on Applied Chemistry 2021, ISAC 2021
CityVirtual, Online
Period28/09/2130/09/21

Keywords

  • chitosan
  • L. casei
  • nano chitin

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