TY - JOUR
T1 - The motility of Tor soro fish (Valenciennes, 1842) using post cryopreservation sperm
T2 - 1st International Conference on Fisheries and Marine, ICFM 2020
AU - Anabella, N. A.
AU - Abinawanto, A.
AU - Subagja, J.
AU - Arifin, O. Z.
AU - Muhiardi, I.
AU - Arief, M. Z.
N1 - Funding Information:
This research was supported by the Directorate of Research and Community Service, University of Indonesia (HIBAH PIT 9 2019: NKB-0015/UN2.R3.1/HKP.05.00/2019) on behalf of Dr. Abinawanto and Installations for Freshwater Fish Genetic Resources, Ministry of Marine Affairs and Fisheries Cijeruk, West Java, Indonesia.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - Tor soro is an endemic fish found on several islands in Indonesia including Java, Sulawesi and Kalimantan. Tor soro has decreased in number due to overfishing, habitat destruction and asynchronous maturation time of male and female gonad. One of the alternative solutions to solve the problem is sperm cryopreservation. Therefore, the objective of this study is to evaluate the effect of grape juice as a natural antioxidant on sperm motility of Tor soro 24 hours post cryopreservation. The sperm was collected through stripping method, and was diluted using 1:9 comparison into the soluble liquid (Fish Ringer + DMSO 10% + grape juice). The concentrations of the grape juice were 0%, 10%, 20%, and 30%, respectively. The equilibration was carried out at 4°:C for 3 h, sample were then frozen at -196°:C for 24 h in liquid nitrogen and were thawed at 40°:C for 1 min. The sperm motility percentage was analyzed using ANOVA and was followed by Tukey test. The result showed that there was an effect (p<0.05) of grape juice on the post cryopreservation spermatozoa motility. The 10% grape juice showed the highest sperm motility (41,8 ± 11,2 %) 24 h post cryopreservation.
AB - Tor soro is an endemic fish found on several islands in Indonesia including Java, Sulawesi and Kalimantan. Tor soro has decreased in number due to overfishing, habitat destruction and asynchronous maturation time of male and female gonad. One of the alternative solutions to solve the problem is sperm cryopreservation. Therefore, the objective of this study is to evaluate the effect of grape juice as a natural antioxidant on sperm motility of Tor soro 24 hours post cryopreservation. The sperm was collected through stripping method, and was diluted using 1:9 comparison into the soluble liquid (Fish Ringer + DMSO 10% + grape juice). The concentrations of the grape juice were 0%, 10%, 20%, and 30%, respectively. The equilibration was carried out at 4°:C for 3 h, sample were then frozen at -196°:C for 24 h in liquid nitrogen and were thawed at 40°:C for 1 min. The sperm motility percentage was analyzed using ANOVA and was followed by Tukey test. The result showed that there was an effect (p<0.05) of grape juice on the post cryopreservation spermatozoa motility. The 10% grape juice showed the highest sperm motility (41,8 ± 11,2 %) 24 h post cryopreservation.
UR - http://www.scopus.com/inward/record.url?scp=85096354942&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/584/1/012063
DO - 10.1088/1755-1315/584/1/012063
M3 - Conference article
AN - SCOPUS:85096354942
SN - 1755-1307
VL - 584
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012063
Y2 - 13 July 2020 through 14 July 2020
ER -