TY - JOUR
T1 - The molecular and clinical features of dengue during outbreak in Jambi, Indonesia in 2015
AU - Haryanto, Sotianingsih
AU - Hayati, Rahma F.
AU - Yohan, Benediktus
AU - Sijabat, Lanceria
AU - Sihite, Ifo F.
AU - Fahri, Sukmal
AU - Meutiawati, Febrina
AU - Halim, Jonathan A.N.
AU - Halim, Stefanie N.
AU - Kusumo, Amin Subandrio W.
AU - Sasmono, R. Tedjo
PY - 2016/4/2
Y1 - 2016/4/2
N2 - Dengue is hyperendemic in Indonesia. In 2015, reported cases of dengue fever doubled those of 2014 in the Jambi municipality of Sumatra. We examined viral aetiology and its relationship with disease outcome in Jambi. Dengue-suspected patients’ sera were collected and NS1 detection and IgM/IgG serology were performed. Dengue virus (DENV) serotyping was performed using real-time RT-PCR. Envelope genes were sequenced to determine the genotypes of DENV. Clinical, haematologic, and demographic data were recorded. Of 210 dengue-suspected patients, 107 were confirmed. The disease manifested as Dengue Fever (62%), Dengue Haemorrhagic Fever (36%), and Dengue Shock Syndrome (2%). The serotypes of 94 DENV were determined. All DENV serotypes were detected with DENV-1 as the predominant serotype (66%). Genotypically, the DENV-1 viruses belong to Genotype I, DENV-2 was of Cosmopolitan genotype, DENV-3 as Genotype I, and DENV-4 belonged to Genotype II. Comparison with historical data revealed serotype predominance switched from DENV-3 to DENV-1, and the replacement of Genotype IV of DENV-1 with Genotype I. In summary, DENV-1 predominated during the 2015 dengue outbreak in Jambi. The full spectrum of dengue disease occurred and was characterized by a switch in predominant serotypes.
AB - Dengue is hyperendemic in Indonesia. In 2015, reported cases of dengue fever doubled those of 2014 in the Jambi municipality of Sumatra. We examined viral aetiology and its relationship with disease outcome in Jambi. Dengue-suspected patients’ sera were collected and NS1 detection and IgM/IgG serology were performed. Dengue virus (DENV) serotyping was performed using real-time RT-PCR. Envelope genes were sequenced to determine the genotypes of DENV. Clinical, haematologic, and demographic data were recorded. Of 210 dengue-suspected patients, 107 were confirmed. The disease manifested as Dengue Fever (62%), Dengue Haemorrhagic Fever (36%), and Dengue Shock Syndrome (2%). The serotypes of 94 DENV were determined. All DENV serotypes were detected with DENV-1 as the predominant serotype (66%). Genotypically, the DENV-1 viruses belong to Genotype I, DENV-2 was of Cosmopolitan genotype, DENV-3 as Genotype I, and DENV-4 belonged to Genotype II. Comparison with historical data revealed serotype predominance switched from DENV-3 to DENV-1, and the replacement of Genotype IV of DENV-1 with Genotype I. In summary, DENV-1 predominated during the 2015 dengue outbreak in Jambi. The full spectrum of dengue disease occurred and was characterized by a switch in predominant serotypes.
KW - Dengue
KW - Genotype
KW - Indonesia
KW - Jambi
KW - Serotype
UR - http://www.scopus.com/inward/record.url?scp=84982282597&partnerID=8YFLogxK
U2 - 10.1080/20477724.2016.1184864
DO - 10.1080/20477724.2016.1184864
M3 - Article
C2 - 27215933
AN - SCOPUS:84982282597
VL - 110
SP - 119
EP - 129
JO - Pathogens and Global Health
JF - Pathogens and Global Health
SN - 2047-7724
IS - 3
ER -