TY - JOUR
T1 - The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing
AU - Abinawanto, null
AU - Fitrianingrum, Nisa
AU - Lestari, Retno
AU - Sudaryono, Agung
AU - Rostika, Rita
AU - Fujaya, Yushinta
PY - 2015
Y1 - 2015
N2 - The spermatozoa quality of goramy two days after sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for two days. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.
AB - The spermatozoa quality of goramy two days after sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for two days. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.
U2 - 10.21534/ai.v16i1
DO - 10.21534/ai.v16i1
M3 - Article
SN - 0216-0749
VL - 16
JO - Aquacultura Indonesiana
JF - Aquacultura Indonesiana
IS - 1
ER -