The initial dioxigenase gene squences analysis of marine bacteria strain M128

Muhamad Sahlan; Hanif Yuliani; Heri Hermansyah; Anondho Wijanarko

doi:10.22146/ajche.49723

The initial dioxigenase gene squences analysis of marine bacteria strain M128

Muhamad Sahlan, Hanif Yuliani, Heri Hermansyah, Anondho Wijanarko

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzedby multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAcgene amplification. The nahAcgene commonly used as biomarkers of PAH degradation, and as a result, nahAcgene sequence analysis of marine bacteria M128strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.

Original language	English
Pages (from-to)	33-42
Number of pages	10
Journal	ASEAN Journal of Chemical Engineering
Volume	13
Issue number	1
DOIs	https://doi.org/10.22146/ajche.49723
Publication status	Published - 2013

Keywords

Analysis
Biodegradation
Dioxygenase
Gene sequence
PAHs

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10.22146/ajche.49723

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abstract = "Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzedby multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAcgene amplification. The nahAcgene commonly used as biomarkers of PAH degradation, and as a result, nahAcgene sequence analysis of marine bacteria M128strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.",

keywords = "Analysis, Biodegradation, Dioxygenase, Gene sequence, PAHs",

author = "Muhamad Sahlan and Hanif Yuliani and Heri Hermansyah and Anondho Wijanarko",

year = "2013",

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T1 - The initial dioxigenase gene squences analysis of marine bacteria strain M128

AU - Sahlan, Muhamad

AU - Yuliani, Hanif

AU - Hermansyah, Heri

AU - Wijanarko, Anondho

PY - 2013

Y1 - 2013

N2 - Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzedby multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAcgene amplification. The nahAcgene commonly used as biomarkers of PAH degradation, and as a result, nahAcgene sequence analysis of marine bacteria M128strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.

AB - Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzedby multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAcgene amplification. The nahAcgene commonly used as biomarkers of PAH degradation, and as a result, nahAcgene sequence analysis of marine bacteria M128strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.

KW - Analysis

KW - Biodegradation

KW - Dioxygenase

KW - Gene sequence

KW - PAHs

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JF - ASEAN Journal of Chemical Engineering

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The initial dioxigenase gene squences analysis of marine bacteria strain M128

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