The influence of transglutaminase enzyme dosage on mixture of soy protein powder and texturized soy protein (TSP) into analog nutritional vegetable meat

Setiadi, Nadia Alisha, Sauria Karina

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The essential amino acids contain in vegetable and animal protein is important for human nutrition intake. However, the presence of the animal protein in the animal meat generally exists with a lot of fat and cholesterol type Low Density Lipoprotein (LDL) that can cause a certain disease. Vegetable protein should be the alternative consumption to decrease the LDL cholesterol content. The current growing technology, the vegetable protein could be restructured to resemble the texture of animal meat. One of the vegetable protein sources that has the same nutritional quality similar to animal meat is soybean protein. This study aims to determine the effect of transglutaminase (TG-ase) enzyme as a crosslinking agent on mixture of Texturized Soy Protein (TSP) and soybean powder. The sample would be examined by acidity level test, function group, texture profile, organoleptic and proximate analysis. By these tests, it could be obtained the optimum of amount transgluminase dosage and the incubation temperature. The experimental variations were the enzyme dosage (0.0%; 0.5%, 1.0%; 1.5%; 2%) and the incubation temperature (5°C; 15 °C; 25°C) with the duration of 24h incubation. The result showed that the increased dosage of transglutaminase enzyme affects the texture of sample. While, enzymatic reaction evidenced by FTIR and acidity level test. In this research the optimum enzyme is 1.5% and the optimum temperature is 25°C. However, the characteristic of this temperature variation wasn't significantly different.

Original languageEnglish
Title of host publication11th Regional Conference on Chemical Engineering, RCChE 2018
EditorsTeguh Ariyanto, Imam Prasetyo, Rochmadi, Nur Rofiqoh Eviana Putri
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418158
DOIs
Publication statusPublished - 21 Mar 2019
Event11th Regional Conference on Chemical Engineering, RCChE 2018 - Yogyakarta, Indonesia
Duration: 7 Nov 20188 Nov 2018

Publication series

NameAIP Conference Proceedings
Volume2085
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference11th Regional Conference on Chemical Engineering, RCChE 2018
CountryIndonesia
CityYogyakarta
Period7/11/188/11/18

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