TY - GEN
T1 - The influence of heating process on anticancer activity of Pterois volitans (red lionfish) venom extraction against human cervical carcinoma cell
AU - Sommeng, Andy Noorsaman
AU - Sari, Mustika
AU - Ginting, Mikael Januardi
AU - Sahlan, Muhamad
AU - Hermansyah, Heri
AU - Wijanarko, Anondho
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Lionfish (Pterois volitans) is an invasive species originating from Indo-Pacific Sea. The rapid growth and the absence of natural predators cause Lionfish to breed rapidly and reduce the native fish populations by up to 80%. Therefore, the study of the Lionfish must be intensified to obtain the usefulness of these animals. Studies that have stated that the extract of poison lionfish spines has the potential to become an anticancer agent for cervical cancer. The aim of the present work is to Investigation the Anticancer activity of Pterois volitans from Indonesia to human cervical carcinoma cell (HeLa cell). Crude venom (CV) that is resulted from the extraction process by sonicating of fresh venomous Pterois volitans as a sample was used. The PLA 2 sample of crude venom was heated on (50, 55, 60, 65, 70) °C for 30 min and purified by (20, 40, 60, 80) % ammonium sulfate. The concentration and purity are analyzed using Lowry method, Marinette's, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and toxicity tested using the BSLT method (Brine Shrimp Lethality Test). The results of sample testing showed that the sample purified with 80% ammonium sulfate at 70°C had the highest protein activity and purity. After determining the purest and safest sample, the chosen sample then tested into HeLLa cell at 2, 250, 500, and 750 ppm, and compared to the cell control sample. The result has shown that the sample 80% of ammonium sulfate at 70°C is highest inhibition level toward HeLLa cell culture at 750 ppm.
AB - Lionfish (Pterois volitans) is an invasive species originating from Indo-Pacific Sea. The rapid growth and the absence of natural predators cause Lionfish to breed rapidly and reduce the native fish populations by up to 80%. Therefore, the study of the Lionfish must be intensified to obtain the usefulness of these animals. Studies that have stated that the extract of poison lionfish spines has the potential to become an anticancer agent for cervical cancer. The aim of the present work is to Investigation the Anticancer activity of Pterois volitans from Indonesia to human cervical carcinoma cell (HeLa cell). Crude venom (CV) that is resulted from the extraction process by sonicating of fresh venomous Pterois volitans as a sample was used. The PLA 2 sample of crude venom was heated on (50, 55, 60, 65, 70) °C for 30 min and purified by (20, 40, 60, 80) % ammonium sulfate. The concentration and purity are analyzed using Lowry method, Marinette's, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and toxicity tested using the BSLT method (Brine Shrimp Lethality Test). The results of sample testing showed that the sample purified with 80% ammonium sulfate at 70°C had the highest protein activity and purity. After determining the purest and safest sample, the chosen sample then tested into HeLLa cell at 2, 250, 500, and 750 ppm, and compared to the cell control sample. The result has shown that the sample 80% of ammonium sulfate at 70°C is highest inhibition level toward HeLLa cell culture at 750 ppm.
KW - anticancer activity
KW - phospholipase A2
KW - Pterois volitans
UR - http://www.scopus.com/inward/record.url?scp=85076755493&partnerID=8YFLogxK
U2 - 10.1063/1.5139359
DO - 10.1063/1.5139359
M3 - Conference contribution
AN - SCOPUS:85076755493
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -