The influence of heating process on anticancer activity of Pterois volitans (red lionfish) venom extraction against human cervical carcinoma cell

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Abstract

Lionfish (Pterois volitans) is an invasive species originating from Indo-Pacific Sea. The rapid growth and the absence of natural predators cause Lionfish to breed rapidly and reduce the native fish populations by up to 80%. Therefore, the study of the Lionfish must be intensified to obtain the usefulness of these animals. Studies that have stated that the extract of poison lionfish spines has the potential to become an anticancer agent for cervical cancer. The aim of the present work is to Investigation the Anticancer activity of Pterois volitans from Indonesia to human cervical carcinoma cell (HeLa cell). Crude venom (CV) that is resulted from the extraction process by sonicating of fresh venomous Pterois volitans as a sample was used. The PLA 2 sample of crude venom was heated on (50, 55, 60, 65, 70) °C for 30 min and purified by (20, 40, 60, 80) % ammonium sulfate. The concentration and purity are analyzed using Lowry method, Marinette's, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and toxicity tested using the BSLT method (Brine Shrimp Lethality Test). The results of sample testing showed that the sample purified with 80% ammonium sulfate at 70°C had the highest protein activity and purity. After determining the purest and safest sample, the chosen sample then tested into HeLLa cell at 2, 250, 500, and 750 ppm, and compared to the cell control sample. The result has shown that the sample 80% of ammonium sulfate at 70°C is highest inhibition level toward HeLLa cell culture at 750 ppm.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/TerritoryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • anticancer activity
  • phospholipase A2
  • Pterois volitans

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