TY - JOUR
T1 - The influence of carboxylate moieties for efficient loading and pH-controlled release of doxorubicin in Fe3O4 magnetic nanoparticles
AU - Saepudin, Endang
AU - Fadhilah, Hasna Resti
AU - Khalil, Munawar
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10/5
Y1 - 2020/10/5
N2 - One of the significant challenges in a nanoparticles-based anticancer drug delivery system for cancer therapy application is to design nanoparticles with specific functionality for efficient drug conjugation and release. In the present study, different carboxylate molecules, i.e., succinic acid (SA), ascorbic acid (AA), and citric acid (CA), were used as surface modifiers for delivery of an anticancer drug, doxorubicin (DOX), using Fe3O4 magnetic nanoparticles (MNPs). Here, a simple one-pot co-precipitation method was successfully used to fabricate monodisperse and uniform carboxylates functionalized Fe3O4 nanoparticles with excellent superparamagnetic properties and colloidal stabilities. Based on the result, the as-prepared MNPs exhibited high drug loading due to the formation of intermolecular forces between DOX and carboxylates moieties or MNPs surface. Besides, the release of the loaded DOX could quickly be released by simply reducing the pH of the suspension. However, it was observed that both loading efficiency and release of DOX were significantly affected by the type of carboxylate moieties. Finally, in vitro MTT assay of HeLa cell lines also revealed that the as-prepared DOX-loaded nanoparticles were able to induce the death of the cancer cell efficiently.
AB - One of the significant challenges in a nanoparticles-based anticancer drug delivery system for cancer therapy application is to design nanoparticles with specific functionality for efficient drug conjugation and release. In the present study, different carboxylate molecules, i.e., succinic acid (SA), ascorbic acid (AA), and citric acid (CA), were used as surface modifiers for delivery of an anticancer drug, doxorubicin (DOX), using Fe3O4 magnetic nanoparticles (MNPs). Here, a simple one-pot co-precipitation method was successfully used to fabricate monodisperse and uniform carboxylates functionalized Fe3O4 nanoparticles with excellent superparamagnetic properties and colloidal stabilities. Based on the result, the as-prepared MNPs exhibited high drug loading due to the formation of intermolecular forces between DOX and carboxylates moieties or MNPs surface. Besides, the release of the loaded DOX could quickly be released by simply reducing the pH of the suspension. However, it was observed that both loading efficiency and release of DOX were significantly affected by the type of carboxylate moieties. Finally, in vitro MTT assay of HeLa cell lines also revealed that the as-prepared DOX-loaded nanoparticles were able to induce the death of the cancer cell efficiently.
KW - Doxorubicin
KW - Drug delivery
KW - FeO nanoparticles
KW - Surface functionalization
UR - http://www.scopus.com/inward/record.url?scp=85086640143&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2020.125137
DO - 10.1016/j.colsurfa.2020.125137
M3 - Article
AN - SCOPUS:85086640143
SN - 0927-7757
VL - 602
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 125137
ER -