TY - JOUR
T1 - The healing effect of adipose-derived stem cell conditioned medium on burn wound model
AU - Eryani, Astheria
AU - Sukmawati, Dewi
AU - Damayanti, Lia
AU - Angmalisang, Elvin Clara
AU - Pawitan, Jeanne A.
N1 - Publisher Copyright:
© Society for Biomaterials & Artificial Organs, India
PY - 2018/7/25
Y1 - 2018/7/25
N2 - Adipose-derived stem cells conditioned medium (ADSC-CM) was harvested from ADSC culture, which is known to contain cytokines and growth factors as regenerative agents. This study aim is to investigate the effect of ADSC-CM local application in promoting burn wound healing. Male Sprague dawley rats were randomly divided into control, medium complete (MC) and conditioned medium (CM) groups. Burn wounds were created by placing a preheated metal plate on the dorsal of thorax. The wounds were then received topical treatment according to the groups. Skin wound tissues were collected in time course and processed by paraffin method. The wounds were evaluated based on macroscopic observation, percentage of wound closure (digital visitrak) and microscopic observation (HE and Masson’s trichrome). ADSC-CM local applications showed to enhance wound closure by reducing the wound area compared to control (p<0.05). It also promoted the healing process better than control group as shown in epithelialization, collagen formation and distribution of granulation tissue. The wound closure in ADSC-CM group was prominent in day 12 and 18 after wounded whereas other groups had longer time. In conclusion, our findings suggest the benefits of ADSC-CM in promoting burn wound healing through acceleration of wound closure and skin regeneration.
AB - Adipose-derived stem cells conditioned medium (ADSC-CM) was harvested from ADSC culture, which is known to contain cytokines and growth factors as regenerative agents. This study aim is to investigate the effect of ADSC-CM local application in promoting burn wound healing. Male Sprague dawley rats were randomly divided into control, medium complete (MC) and conditioned medium (CM) groups. Burn wounds were created by placing a preheated metal plate on the dorsal of thorax. The wounds were then received topical treatment according to the groups. Skin wound tissues were collected in time course and processed by paraffin method. The wounds were evaluated based on macroscopic observation, percentage of wound closure (digital visitrak) and microscopic observation (HE and Masson’s trichrome). ADSC-CM local applications showed to enhance wound closure by reducing the wound area compared to control (p<0.05). It also promoted the healing process better than control group as shown in epithelialization, collagen formation and distribution of granulation tissue. The wound closure in ADSC-CM group was prominent in day 12 and 18 after wounded whereas other groups had longer time. In conclusion, our findings suggest the benefits of ADSC-CM in promoting burn wound healing through acceleration of wound closure and skin regeneration.
UR - http://www.scopus.com/inward/record.url?scp=85054960657&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85054960657
SN - 0971-1198
VL - 32
SP - 18
EP - 25
JO - Trends in Biomaterials and Artificial Organs
JF - Trends in Biomaterials and Artificial Organs
IS - 1
ER -