The growth of Synechococcus sp RDB001 at temperature of 30 ± 5 °c and 50 ± 5 °c: A comparison study of cell density and chlorophyll content

N. B. Prihantini, R. D. Anggriary, L. R. Kusmadji

Research output: Contribution to journalConference article

Abstract

Research on comparison of cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C has been carried out. Synechococcus sp. RDB001 was isolated from hot spring water samples in the area of Rawa Danau-Banten with a water temperature of 50 °C which grown in MA medium (pH 6). The research aims to determine the comparison of the average cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C in incubation cabinets. The research results are useful for understanding the physiological tolerance limits and adaptation of Synechococcus sp. RDB001 ex-situ. Culturing of Synechococcus sp. RDB001 was carried out in cabinets with the incubation temperature of 30 ± 5 °C and 50 ± 5 °C for 16 days, from day 0 (t0) until day 16 (t16). Each treatment was done in 16 replicates. Non-parametric statistical analysis using the Mann Whitney test (α = 0.05) and the Spearman test (α = 0,01). The results showed there were significant differences (α = 0.05) in cell density of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C. The highest average of cell density of Synechococcus sp. RDB001 at a temperature of 30 ± 5 °C occurred at t12, (24.2075 ± 5.33926 x 106 cells.mL-1), while at a temperature of 50 ± 5 °C occurred at t14 (1.21313 ± 2.92573 x 106 cells.mL-1). There was no correlation (α = 0.01) between cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C. The research showed that increasing the cell density of Synechococcus sp. RDB001 is not always followed by an increase in chlorophyll content of Synechococcus sp. RDB001.

Original languageEnglish
Article number012065
JournalJournal of Physics: Conference Series
Volume1442
Issue number1
DOIs
Publication statusPublished - 29 Jan 2020
EventBasic and Applied Sciences Interdisciplinary Conference 2017, BASIC 2017 - , Indonesia
Duration: 18 Aug 201719 Aug 2017

Keywords

  • cyanobacteria
  • growth temperature
  • hot spring
  • Synechococcus

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