The growth differences between Leptolyngbya HS-16 isolated from Gunung Pancar hot spring (69°C) and Leptolyngbya HS-36 isolated from Maribaya hot spring (42°C) incubated in 20°C and 50°C

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Abstract

The growth differences between cyanobacteria strains Leptolyngbya HS-16 and HS-36, which were incubated in temperatures 20°C, and 50°C had been studied. Those strains were isolated from Gunung Pancar (Leptolyngbya HS-16) and Maribaya (Leptolyngbya HS-36) hot springs which were in West Java, Indonesia. The water temperature of habitats was 69°C (Gunung Pancar) and 42°C (Maribaya). Those strains were grown in batch culture for 7 days in BG-11 medium. Biomass weight during growing of strains Leptolyngbya HS-16 and HS-36 was the parameter of the growth. Observations were done from day-0 (t0) until day-7 (t7). The results showed that there were differences in growth curve of those strains. Biomass weight in day-2 (t2) of Leptolyngbya HS-16 incubated in 20°C was higher than biomass weight in day-0 (t0), however biomass weight in seven days of Leptolyngbya HS-16 incubated in 50°C was lower than biomass weight of day-0 (t0). Biomass weight of Leptolyngbya HS-36 incubated in 20°C was lower than biomass weight of day-0 (t0). Biomass weight in day-3 (t3) of Leptolyngbya HS-36 incubated in 50°C higher than biomass weight in day-0 (t0). Leptolyngbya HS-16 and Leptolyngbya HS-36 that were incubated in 20°C and 50°C went through adaptation (lag) phase. The color of Leptolyngbya HS-16 culture, which was incubated in 20°C, changed from emerald green color (t0) into leaf green color (t7). It also happened at the color of Leptolyngbya HS-36 culture, which was incubated in 50°C it changed from brown ochre color (t0) to nougat color (t7). Growth curve data showed the highest rate of growth in lag phase of Leptolyngbya HS-16 was in 20°C and Leptolyngbya HS-36 was in 50°C, but their color of cultures were changed because of the metabolic reaction.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

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    Pertiwi, Z. D., & Prihantini, N. B. (2018). The growth differences between Leptolyngbya HS-16 isolated from Gunung Pancar hot spring (69°C) and Leptolyngbya HS-36 isolated from Maribaya hot spring (42°C) incubated in 20°C and 50°C. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020125] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064122