TY - JOUR
T1 - The Expressions of NF−κB, COX−2, Sp1, and c−Jun in Pancreatic Ductal Adenocarcinoma and Their Associations with Patient Survival
AU - Renaldi, Kaka
AU - Simadibrata, Marcellus
AU - Rahadiani, Nur
AU - Handjari, Diah Rini
AU - Harahap, Alida Roswita
AU - Harimurti, Kuntjoro
AU - Zubir, Nasrul
AU - Siregar, Lianda
AU - Loho, Imelda Maria
AU - Suzanna, Evlina
AU - Prawirodihardjo, Bonita
AU - Hidajat, Heriawaty
AU - Widodo, Budi
AU - Rahniayu, Alphania
AU - Tambun, Renaningtyas
AU - William, Andy
AU - Makmun, Dadang
N1 - Funding Information:
This research was funded by the Directorate of Research and Development, Universitas Indonesia, under Hibah PUTI 2022 (grant no. NKB−1589/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/6
Y1 - 2023/6
N2 - Chronic inflammation is a crucial driver of carcinogenesis in pancreatic ductal adenocarcinoma (PDAC). Several studies have investigated the prognostic significance of cyclooxygenase−2 (COX−2) expression in PDAC patients, obtaining conflicting results. Nuclear factor kappa−B (NF−κB), specificity protein 1 (Sp1), and c−Jun are known as the transcription factors of the COX2 gene. This exploratory observational study investigated the association of the NF−κB, COX−2, Sp1, and c−Jun expressions with patient survival in PDAC. We used the immunohistochemical method to detect the PDAC tissue expressions of NF−κB (RelA/p65), COX−2, Sp1, and c−Jun. The expressions of these proteins were correlated with the overall survival (OS) and other clinicopathological characteristics of PDAC patients. We obtained 53 PDAC specimens from resections and biopsies. There were significant correlations between the four proteins’ expressions in the PDAC tissues. The expression of the cytoplasmic (aHR = 0.31; 95% CI 0.11–0.90; p = 0.032) or nuclear NF−κB (aHR = 0.22; 95% CI 0.07–0.66; p = 0.007) was independently associated with a better prognosis in the PDAC patients. COX−2, Sp1, and c−Jun showed no significant association with a prognosis in the PDAC patients. The PDAC patients who expressed NF−κB had a better prognosis than the other patients, which suggests that the role of inflammation in PDAC is more complex than previously thought.
AB - Chronic inflammation is a crucial driver of carcinogenesis in pancreatic ductal adenocarcinoma (PDAC). Several studies have investigated the prognostic significance of cyclooxygenase−2 (COX−2) expression in PDAC patients, obtaining conflicting results. Nuclear factor kappa−B (NF−κB), specificity protein 1 (Sp1), and c−Jun are known as the transcription factors of the COX2 gene. This exploratory observational study investigated the association of the NF−κB, COX−2, Sp1, and c−Jun expressions with patient survival in PDAC. We used the immunohistochemical method to detect the PDAC tissue expressions of NF−κB (RelA/p65), COX−2, Sp1, and c−Jun. The expressions of these proteins were correlated with the overall survival (OS) and other clinicopathological characteristics of PDAC patients. We obtained 53 PDAC specimens from resections and biopsies. There were significant correlations between the four proteins’ expressions in the PDAC tissues. The expression of the cytoplasmic (aHR = 0.31; 95% CI 0.11–0.90; p = 0.032) or nuclear NF−κB (aHR = 0.22; 95% CI 0.07–0.66; p = 0.007) was independently associated with a better prognosis in the PDAC patients. COX−2, Sp1, and c−Jun showed no significant association with a prognosis in the PDAC patients. The PDAC patients who expressed NF−κB had a better prognosis than the other patients, which suggests that the role of inflammation in PDAC is more complex than previously thought.
KW - cyclooxygenase−2
KW - c−Jun
KW - nuclear factor kappa−B
KW - pancreatic neoplasms
KW - Sp1
UR - http://www.scopus.com/inward/record.url?scp=85163632072&partnerID=8YFLogxK
U2 - 10.3390/pathophysiology30020009
DO - 10.3390/pathophysiology30020009
M3 - Article
AN - SCOPUS:85163632072
SN - 0928-4680
VL - 30
SP - 92
EP - 109
JO - Pathophysiology
JF - Pathophysiology
IS - 2
ER -