TY - JOUR
T1 - The expression of nestin in the induced differentiation into neurons of rat bone marrow mesenchymal stem cells by neurotrophin-3 (NT-3)
AU - Noviantari, Ariyani
AU - Antarianto, Radiana Dhewayani
AU - Rif’ati, Lutfah
AU - Rinendyaputri, Ratih
AU - Zainuri, Masagus
AU - Dany, Frans
N1 - Funding Information:
The authors would like to acknowledge the Head of the Center for Research and Development of Biomedical and Basic Health Technology (CRDBBHT), National Institute of Health Research and Development (NIHRD) and the researchers at the Stem Cell Laboratory and Animal Laboratory, CRDBBHT, NIHRD, Ministry of Health, Republic of Indonesia, for creating a conducive environment for the completion of this study. The authors acknowledge the Head of the Histology Department and the Head of the Histology Laboratory, Faculty of Medicine, Universitas Indonesia, for permission to use their laboratory facilities. The authors acknowledge Professor Irawan Satriotomo, MD, Ph. D., from the University of Florida for valuable discussion and feedback on study results. This study was financially supported by the Ministry of Health, the Republic of Indonesia.
Publisher Copyright:
© 2020 The Authors.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: The aim of this study was to examine the role of NT-3 as a single neurotrophic factor in the expression of nestin in the neural differentiation of MSCs. Methods: MSCs were isolated from rat bone marrow and induced with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 and 14 d (the control was no NT-3). Nestin underwent immunocytochemical analysis on days 7 and 14. Five high-power random fields were documented. Results: A post-hoc analysis using LSD after one-way ANOVA test yielded a statistically significant difference in the percentage of nestin-positive cells in MSCs with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 d compared to the control group (p<0.05). The percentages of nestinpositive cells at concentrations of 20, 25, and 30 ng/ml, and in the control data on day 7 were 14.55±1.26%, 16.20±1.07%, 13.78±1.19%, and 9.81±0.79%, respectively. NT-3 at 25 ng/ml induced the highest MSCs neural differentiation on day 7 and remained constant until day 14. Conclusion: NT-3 plays a role in the early stage of differentiating MSCs from rat bone marrow into neurons, with the optimal concentration being 25 ng/ml.
AB - Objective: The aim of this study was to examine the role of NT-3 as a single neurotrophic factor in the expression of nestin in the neural differentiation of MSCs. Methods: MSCs were isolated from rat bone marrow and induced with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 and 14 d (the control was no NT-3). Nestin underwent immunocytochemical analysis on days 7 and 14. Five high-power random fields were documented. Results: A post-hoc analysis using LSD after one-way ANOVA test yielded a statistically significant difference in the percentage of nestin-positive cells in MSCs with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 d compared to the control group (p<0.05). The percentages of nestinpositive cells at concentrations of 20, 25, and 30 ng/ml, and in the control data on day 7 were 14.55±1.26%, 16.20±1.07%, 13.78±1.19%, and 9.81±0.79%, respectively. NT-3 at 25 ng/ml induced the highest MSCs neural differentiation on day 7 and remained constant until day 14. Conclusion: NT-3 plays a role in the early stage of differentiating MSCs from rat bone marrow into neurons, with the optimal concentration being 25 ng/ml.
KW - Differentiation
KW - Mesenchymal stem cells
KW - Nestin
KW - Neuron
KW - Neurotrophin-3
UR - http://www.scopus.com/inward/record.url?scp=85099646762&partnerID=8YFLogxK
U2 - 10.22159/ijap.2020.v12s3.39472
DO - 10.22159/ijap.2020.v12s3.39472
M3 - Article
AN - SCOPUS:85099646762
SN - 0975-7058
VL - 12
SP - 44
EP - 49
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 3
ER -