The enhancement of phycocyanin yield and purity from Spirulina platensis using freeze-thawing method on various solvents

Dianursanti, Maulina Aini Hafidzah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Phycocyanin is a pigment compound that can be found from Spirulina platensis microalgae. It has been used for food colorant, cosmetic dye and fluorescence reagent for clinical diagnosis. Phycocyanin can be extracted using various procedure that was affected by many factors, one of them is the type of solvent that was used in the extraction process. This research will study the usage of different solvents, using sodium phosphate buffer and calcium chloride. The extraction method used in this research is freeze-thawing. The parameters of this extraction are phycocyanin yield and purity. The obtained result shows a higher yield of phycocyanin from extraction using phosphate buffer, with the value of 35.31 mg/g, and a higher phycocyanin purity from extraction using CaCl2 with the ratio of 2.6.

Original languageEnglish
Title of host publication5th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the 5th International Symposium of Biomedical Engineering, ISBE 2020
EditorsKenny Lischer, Sugeng Supriadi, Siti Fauziyah Rahman, Yudan Whulanza
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735440869
DOIs
Publication statusPublished - 23 Mar 2021
Event5th International Symposium of Biomedical Engineering, ISBE 2020 - Depok, Virtual, Indonesia
Duration: 28 Jul 202029 Jul 2020

Publication series

NameAIP Conference Proceedings
Volume2344
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium of Biomedical Engineering, ISBE 2020
Country/TerritoryIndonesia
CityDepok, Virtual
Period28/07/2029/07/20

Keywords

  • CaCl2
  • freeze-thawing
  • Microalgae
  • Phosphate Buffer
  • Phycocyanin Extraction
  • Sonication
  • Spirulina platensis

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