The efficacy of platelet-rich fibrin lysate (PRF-L) for fibroblast cell proliferation

Risya Dini Marsa, Dini Asrianti, Anggraini Margono

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Background: One of the most distinct signs of irreversible pulpitis is the presence of permanently damaged fibroblasts, which is are the primary components of dental pulp. Regeneration of pulp tissue has been difficult, as the tissue is encased in dentin without and lacks a collateral blood supply except from at the root apical end. Growth factors play an important role to in promoting the healing process. It is acknowledged that Platelet-rich fibrin lysate (PRF-L) release contains growth factors and have has the potential ability to promote healing process of the of damaged fibroblasts. Objectives: To evaluate the proliferation capability of serum-starved fibroblasts by using treated with various concentrations of PRF--L. Methods: Human dermal fibroblasts were divided into two groups: Group I consisted of serum-starved fibroblasts and treated by with various three concentrations of PRF-L (50%, 25%, and 12,.5%). Group II consisted of normal fibroblasts as a positive control. , meanwhile s Serum-starved fibroblasts without any PRF-L treatment served as a negative control. Fibroblast proliferation capability was evaluated after PRF-L application and compared to control groups. Results: Serum-starved fibroblasts treated with inside 25% PRF-L had showed significantly (Pp<0.05) increased proliferation rates when compared to control fibroblasts. Fibroblasts proliferation is was lowest in the 12,.5% group but was not significantly different compared to the other PRF-L treatments with other groups. Conclusions: In this study that A 25% PRF-L treatment provided give an optimal result in fibroblast cell proliferation.

Original languageEnglish
Pages (from-to)809-813
Number of pages5
JournalJournal of International Dental and Medical Research
Issue numberSpecialissue
Publication statusPublished - 2017


  • Fibroblasts
  • Growth factor
  • Platelet-rich fibrin lysate (PRF-L)
  • Serum-starved


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