The efficacy of advanced platelet-rich fibrin (a-prf) on fibroblast cell regeneration

Diananda, Kamizar, Anggraini Margono, Dini Asrianti, Ratna Meydiawati

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


One of the most distinct signs of irreversible pulpitis is the presence of permanently damaged fibroblasts, which are the primary component of dental pulp. The low compliance nature of the tooth affects its ability to self-repair and leads to the disruption of the extracellular matrix. Growth factors area substantial part of cell regeneration. It has been acknowledged that advanced platelet-rich fibrin (A-PRF) can promote the healing process in injured fibroblasts. The Objectives of this study was performed to evaluate the capability of various concentrations of A-PRF. An experimental design was carried out in serum-starved fibroblasts. Methods: Fibroblasts were cultivated for three weeks and divided into two groups. Group I consisted of serum-starved fibroblasts. Group I was divided into three subgroups that were treated by various concentrations of A-PRF (50%, 25%, and 12.5%). Group II consisted of normal fibroblasts, serving as the positive control. Fibroblast proliferation was calculated by the Luna-IITM automated cell counter to evaluate the fibroblasts' ability to regenerate after A-PRF application. PRF was demonstrated to stimulate cell regeneration on serum-starved fibroblasts. As a result, the highest proliferation level was shown in the25% group. It was concluded that A-PRF may be a promising therapeutic autologous biomaterial.

Original languageEnglish
Pages (from-to)789-792
Number of pages4
JournalJournal of International Dental and Medical Research
Issue numberSpecialissue
Publication statusPublished - 2017


  • A-PRF
  • Growth factors
  • Irreversible pulpitis
  • Regenerative endodontic
  • Serum-starved fibroblasts


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