The effects of western diet on sperm quantity and quality and serum hormonal concentration in cynomologus monkey (Macaca fascicularis) injected with testosterone enanthate (TE) plus depot medroxy progesterone acetate (DMPA)⁺⁾

The aim of this study is to elucidate the effects of western diet on sperm quantity and quality and serum hormone in cynomologus monkeys (Macaca fascicularis) injected with testosterone enanthate (TE) plus depotmedroxy progesterone acetate (DMPA). The twenty male monkeys are divided into two groups ten animals each. The Group (I) was fed with the “monkey chow" (9% fat, 13% protein and 78% carbohydrate); the second group (II) was fed with "western" diet (35% fat, 25% protein and 40% carbohydrate) Three months after adaptation period, both groups were injected with 20 mg TE and 25 mg DMPA for 18 weeks, while TE injections continued for another 6 week. The semen for examination of Sperm density and quality using electro-ejaculation was done once every three weeks, and determined by WHO methods. The blood serum for hormone assay was taken once every six weeks. In group/azoospermia reached 100%, while in the group II reached only 70%. In both groups the spermatozoal count were recovered at week 33, but until this investigation terminated on week 39 the level of spermatozoa of group I reached only severe oligozoospermia, and two animals remained severe azoospermie; while group II has returned to normospermia. The quality of sperm during and after treatment in group II was better than group I. Hormone concentration decreased more rapidly in group I than in group II, while the recovery period was slower in group I compared to group II. This investigation concluded that different diet formula caused different results in decreasing the level of sperm quantity and quality, and hormone concentration of M. fascicularis injected with TE in combination with DMPA, being lower in animals fed with monkey chow diet than in animals fed with western diet.