TY - JOUR
T1 - The effects of thidiazuron and 2,4-D on the regeneration of Melastoma malabathricum L. cultured leaves
AU - Putri, F.
AU - Yuniati, R.
AU - Handayani, W.
N1 - Publisher Copyright:
© 2020 IOP Publishing Ltd. All rights reserved.
PY - 2020/4/27
Y1 - 2020/4/27
N2 - Melastoma malabathricum L. has potential for exploitation of its pharmacological and phytoremediation properties. We cultivated M. malabathricum leaves using in vitro cultures in Murashige and Skoog (MS) media supplemented with thidiazuron (TDZ) (0, 0.1 and 0.2 mg.L-1), 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 1, 2 and 3 mg.L-1) or both. We studied the effect of TDZ alone or combined with 2,4-D on the growth and development of cultured leaves. Explants were cultured on solid MS medium supplemented with TDZ (0, 1, 2 and 3 mg.L-1) or 2,4-D (0, 0.1 and 0.2 mg.L-1) or both. All treatments induced callus with different colours and textures. TDZ, 2,4-D or both induced callus formation in approximately 75-95 %, 95-100 % or 45-90 % of explants. Adventitious root was produced in the presence of 0.1 mg L-1 (70 %) and 0.2 mg.L -1 (60 %) 2,4-D. Adventitious shoot formation was initiated in the presence of 1 mg.L-1 (15 %), 2 mg.L-1 (5 %) and 3 mg.L-1 (5 %) TDZ. Callus formation was induced by 0.1 mg.L-1 2,4-D (63 %), 0.2 mg.L -1 2,4-D (50 %), 2 mg.L-1 TDZ (42 %) and 3 mg.L-1 TDZ (50 %), which were higher than other treatments. Callus, adventitious root or adventitious shoot was induced from leaves using 12 different media.
AB - Melastoma malabathricum L. has potential for exploitation of its pharmacological and phytoremediation properties. We cultivated M. malabathricum leaves using in vitro cultures in Murashige and Skoog (MS) media supplemented with thidiazuron (TDZ) (0, 0.1 and 0.2 mg.L-1), 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 1, 2 and 3 mg.L-1) or both. We studied the effect of TDZ alone or combined with 2,4-D on the growth and development of cultured leaves. Explants were cultured on solid MS medium supplemented with TDZ (0, 1, 2 and 3 mg.L-1) or 2,4-D (0, 0.1 and 0.2 mg.L-1) or both. All treatments induced callus with different colours and textures. TDZ, 2,4-D or both induced callus formation in approximately 75-95 %, 95-100 % or 45-90 % of explants. Adventitious root was produced in the presence of 0.1 mg L-1 (70 %) and 0.2 mg.L -1 (60 %) 2,4-D. Adventitious shoot formation was initiated in the presence of 1 mg.L-1 (15 %), 2 mg.L-1 (5 %) and 3 mg.L-1 (5 %) TDZ. Callus formation was induced by 0.1 mg.L-1 2,4-D (63 %), 0.2 mg.L -1 2,4-D (50 %), 2 mg.L-1 TDZ (42 %) and 3 mg.L-1 TDZ (50 %), which were higher than other treatments. Callus, adventitious root or adventitious shoot was induced from leaves using 12 different media.
KW - 2,4-dichlorofenoxyacetid acid
KW - callus
KW - Melastoma malabathricum
KW - Thidiazuron
UR - http://www.scopus.com/inward/record.url?scp=85084755913&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/481/1/012028
DO - 10.1088/1755-1315/481/1/012028
M3 - Conference article
AN - SCOPUS:85084755913
SN - 1755-1307
VL - 481
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012028
T2 - Life and Environmental Sciences Academics Forum 2018, LEAF 2018
Y2 - 1 November 2018
ER -