TY - JOUR
T1 - The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris
AU - Dianursanti,
AU - Siregar, Aisyah Razaanah
AU - Maeda, Yoshiaki
AU - Yoshino, Tomoko
AU - Tanaka, Tsuyoshi
N1 - Funding Information:
Acknowledgements The authors would like to thank Tanaka, Arakaki & Yoshino Laboratory, Biomolecular Engineering & Marine Biotechnology, Department of Biotechnology and Life Sciences, Tokyo University of Agriculture and Technology, for their research facilities and JASSO (Japan Student Service Organization) and PUTI for providing financial aid for this research.
Publisher Copyright:
© 2020. The American Society of Hematology. All Rights Reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/20
Y1 - 2020/11/20
N2 - Research around using algae as a natural source of carotenoids has been intense in the 21st century, given the wide applications of carotenoids in the pharmaceutical, health, and food industries. This study aimed to get the highest yield of carotenoids from Chlorella vulgaris by ultrasound extraction. This study evaluated two parameters: the extraction solvent (ethanol, acetone, and diethyl ether were tested) and the solid-to-solvent ratio (1:30, 1:50, and 1:100 were tested). The carotenoid extracted from C. vulgaris was lutein, and its compounds were identified by UV-Vis spectroscopy. The highest carotenoid yield was achieved using ethanol at 1.146±0.082 mg/g and a solid-to-solvent ratio of 1:100 (g/mL). This research shows the use of a specific extraction solvent along with a solid-to-solvent ratio is significant to determine carotenoids yield desired. Further study of other parameters (e.g., temperature and ultrasound intensity) is necessary for the optimum extraction condition.
AB - Research around using algae as a natural source of carotenoids has been intense in the 21st century, given the wide applications of carotenoids in the pharmaceutical, health, and food industries. This study aimed to get the highest yield of carotenoids from Chlorella vulgaris by ultrasound extraction. This study evaluated two parameters: the extraction solvent (ethanol, acetone, and diethyl ether were tested) and the solid-to-solvent ratio (1:30, 1:50, and 1:100 were tested). The carotenoid extracted from C. vulgaris was lutein, and its compounds were identified by UV-Vis spectroscopy. The highest carotenoid yield was achieved using ethanol at 1.146±0.082 mg/g and a solid-to-solvent ratio of 1:100 (g/mL). This research shows the use of a specific extraction solvent along with a solid-to-solvent ratio is significant to determine carotenoids yield desired. Further study of other parameters (e.g., temperature and ultrasound intensity) is necessary for the optimum extraction condition.
KW - Carotenoids
KW - Chlorella vulgaris
KW - Lutein
KW - Solvent extraction
KW - Ultrasound extraction
UR - http://www.scopus.com/inward/record.url?scp=85097887925&partnerID=8YFLogxK
U2 - 10.14716/ijtech.v11i5.4331
DO - 10.14716/ijtech.v11i5.4331
M3 - Article
AN - SCOPUS:85097887925
SN - 2086-9614
VL - 11
SP - 941
EP - 950
JO - International Journal of Technology
JF - International Journal of Technology
IS - 5
ER -