The effects of heating process on protein isolation of lionfish (Pterois volitans) spines venom extract to antioxidant activity assay

Andy Noorsaman Sommeng, Indriani Pratiwi, Mikael Januardi Ginting, Muhamad Sahlan, Heri Hermansyah, Anondho Wijanarko

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Lionfish (Pterois volitans) was classified as an invasive predator native to the Indo-Pacific region that has no natural predators. Lionfish grow fast, making these fish prey on much smaller fish in which causing damage to the marine ecosystem. This research determines the antioxidant potential of lionfish spines venom by isolating the protein from its extract. In previous research, it was specified that lionfish poison extract have had the potential as an antioxidant, however, it was still feeble. In order to find out the potential further, the additional isolation step is heating. Crude venom (CV) was extracted using sonication and isolated by heating at 90°C, 75°C, and 60°C followed by fractionated using Ammonium Sulfate (AS) with 0-20, 20-40, 40-60, and 60-80% saturation. The concentrated protein was then analyzed by the Lowry test and the protein content was identified using SDS-PAGE, followed by toxicity test using BSLT method (Brine Shrimp Lethality Test). The antioxidant activity was carried out using the DPPH method in the final step. The optimum condition for isolating proteins which have the potential to have antioxidant activity observed in specimen with a heating temperature of 75 and saturation of ammonium sulfate 40-60% with an IC50 value of 1312 ppm. The protein composition at the protein isolation temperature for optimum antioxidant is protein 7.9; 46.2; and 52.7 kD.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/TerritoryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • Antioxidant activity
  • Crude venom
  • Protein isolation

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