TY - JOUR
T1 - The Effects of Folic Acid-conjugated Nanoparticle on Doxorubicin Localization within HeLa Cells and the Effects of Doxorubicin on Chromosome Structure
AU - Damacena, R.
AU - Jesaya, J.
AU - Mualifah, M.
AU - Khalil, M.
AU - Dwiranti, A.
N1 - Publisher Copyright:
© 2024 American Institute of Physics Inc.. All rights reserved.
PY - 2024/9/30
Y1 - 2024/9/30
N2 - Cervical cancer is one of the cancers with the highest incidence rates in Indonesia. One of the treatments commonly used for cervical cancer is chemotherapy using doxorubicin. Doxorubicin is one of the compounds belong to the anthracycline class which is responsible for inhibiting the Topoisomerase II enzyme. The drug delivery technology with nanoparticles has the potential to increase the efficacy of chemotherapy. Nanoparticles (Fe3O4) and folic acid can be used for the development of drug delivery. To date, the information of doxorubicin distribution within HeLa cells using folic acid conjugated nanoparticles has been limited. Furthermore, Topoisomerase II has been reported to be correlated with the chromosome structure. Nevertheless, the information about the effects of doxorubicin on chromosome structure has yet to be revealed. This study aimed to evaluate the effects of folic acid conjugation on nanoparticles on the internalization of doxorubicin and to evaluate the effects of doxorubicin on the HeLa chromosome. The HeLa cells were treated with doxorubicin, doxorubicin with the nanoparticle, and doxorubicin with folic acid-conjugated nanoparticles. Doxorubicin distribution within the cell was visualized using a fluorescence microscope. For chromosome investigation, the HeLa cell was cultured and treated with 4 µg/mL doxorubicin. Chromosomes were stained with Giemsa and observed. The results obtained in this study showed that the number of cells treated with doxorubicin with folic acid-conjugated nanoparticles was less than the control. Furthermore, the number, length, and area of the control chromosomes were 41-75 chromosomes/cell, 2-5 µm, and 4-15 µm2, respectively. On the contrary, the metaphase chromosome from the cells treated with doxorubicin could not be obtained. These results further strengthen that the folic acid helped the cells to internalize doxorubicin and that the doxorubicin inhibits the activity of Topoisomerase II.
AB - Cervical cancer is one of the cancers with the highest incidence rates in Indonesia. One of the treatments commonly used for cervical cancer is chemotherapy using doxorubicin. Doxorubicin is one of the compounds belong to the anthracycline class which is responsible for inhibiting the Topoisomerase II enzyme. The drug delivery technology with nanoparticles has the potential to increase the efficacy of chemotherapy. Nanoparticles (Fe3O4) and folic acid can be used for the development of drug delivery. To date, the information of doxorubicin distribution within HeLa cells using folic acid conjugated nanoparticles has been limited. Furthermore, Topoisomerase II has been reported to be correlated with the chromosome structure. Nevertheless, the information about the effects of doxorubicin on chromosome structure has yet to be revealed. This study aimed to evaluate the effects of folic acid conjugation on nanoparticles on the internalization of doxorubicin and to evaluate the effects of doxorubicin on the HeLa chromosome. The HeLa cells were treated with doxorubicin, doxorubicin with the nanoparticle, and doxorubicin with folic acid-conjugated nanoparticles. Doxorubicin distribution within the cell was visualized using a fluorescence microscope. For chromosome investigation, the HeLa cell was cultured and treated with 4 µg/mL doxorubicin. Chromosomes were stained with Giemsa and observed. The results obtained in this study showed that the number of cells treated with doxorubicin with folic acid-conjugated nanoparticles was less than the control. Furthermore, the number, length, and area of the control chromosomes were 41-75 chromosomes/cell, 2-5 µm, and 4-15 µm2, respectively. On the contrary, the metaphase chromosome from the cells treated with doxorubicin could not be obtained. These results further strengthen that the folic acid helped the cells to internalize doxorubicin and that the doxorubicin inhibits the activity of Topoisomerase II.
KW - Chromosome
KW - doxorubicin
KW - folic acid
KW - HeLa cells
KW - magnetite nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=85206802506&partnerID=8YFLogxK
U2 - 10.1063/5.0214352
DO - 10.1063/5.0214352
M3 - Conference article
AN - SCOPUS:85206802506
SN - 0094-243X
VL - 3163
JO - AIP Conference Proceedings
JF - AIP Conference Proceedings
IS - 1
M1 - 070003
T2 - 7th International Symposium on Current Progress in Mathematics and Sciences 2021, ISCPMS 2021
Y2 - 6 October 2021 through 7 October 2021
ER -