Binucleate Rhizoctonia (BNR) fungi are essential for the germination of vanilla seeds. Chemical control of the soil-borne pathogen might adversely affect BNR. The purpose of this study is to determine the effect of Nicotiana tabacum extract biopesticides and Fusarium oxysporum f.sp. vanillae (Fusarium) on vanilla plant nutrient content induced by BNR. Materials and Methods. The research design was completely randomized design with two factors and three replications. The first factor was biopesticide (dosage of 0, 10, 20, and 30 ml/seedling), and the second factor was the application of Fusarium. Results. The increase in the nitrogen, phosphorus, and potassium content of vanilla was affected by biopesticides and Fusarium inoculation. Fusarium inoculation has no significant effect on nitrogen and phosphorus levels but significantly affects potassium levels. The biopesticide dosage is significant for nitrogen, phosphorus, and potassium. The interaction of biopesticides with Fusarium inoculation did not significantly affect the parameters of nitrogen and phosphorus content, but significantly affected potassium content. Conclusion. The application of biopesticides and Fusarium inoculation after induction of BNR can increase nitrogen, phosphorus, and potassium content of vanilla plants.