TY - JOUR
T1 - The effects of apple juice (Malus sylverstris-Mill) as a natural antioxidant on spermatozoa viability of Tor soro 24 hours postcryopreservation
AU - Arief, M. Z.
AU - Anabella, N. A.
AU - Muhiardi, I.
AU - Abinawanto,
AU - Arifin, O. Z.
N1 - Funding Information:
This work was supported by Universitas Indonesia (under HIBAH PIT 9 of the year 2019 with contract number NKB.0015/UN2.R31/HKP.05.00/2019 on behalf of Dr. Abinawanto), and Installations for Freshwater Fish Genetic Resources, Ministry of Marine Affairs and Fisheries, Cijeruk, West Java, Indonesia.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/10/4
Y1 - 2021/10/4
N2 - The population of Tor soro in Indonesia continues to decline due to overfishing. In T. soro farming, the gonad maturity between males and females tends to be asynchronous. Thus, it is necessary to have a stock of milt that can be used at any time for fertilization of the eggs by cryopreservation. Cryopreservation can cause cell damage due to oxidative stress and can be reduced by the addition of antioxidants in the cryopreservation medium. Apple (Malus sylverstris-Mill) can act as an additional antioxidant to increase the chance of spermatozoa survival post-cryopreservation. This study aims to study the influence of adding various concentrations of apple juice (0 %, 10 %, 20 %, and 30 %) and determine which is most optimal in maintaining the viability of T. soro spermatozoa 24 hours post-cryopreservation with a 1:9 milt-diluent ratio. The mean viability of T. soro spermatozoa post-cryopreservation in 0 %, 10 %, 20 %, and 30 % concentrations are 12.01 ? 2.24 %, 8.10 ? 4.53 %, 53 ? 5.14 %, and 6.23 ? 4 % respectively. The results of the one-way ANOVA test showed that the apple juice in various concentrations did not affect the viability of T. soro spermatozoa 24 hours post-cryopreservation (P>0.5).
AB - The population of Tor soro in Indonesia continues to decline due to overfishing. In T. soro farming, the gonad maturity between males and females tends to be asynchronous. Thus, it is necessary to have a stock of milt that can be used at any time for fertilization of the eggs by cryopreservation. Cryopreservation can cause cell damage due to oxidative stress and can be reduced by the addition of antioxidants in the cryopreservation medium. Apple (Malus sylverstris-Mill) can act as an additional antioxidant to increase the chance of spermatozoa survival post-cryopreservation. This study aims to study the influence of adding various concentrations of apple juice (0 %, 10 %, 20 %, and 30 %) and determine which is most optimal in maintaining the viability of T. soro spermatozoa 24 hours post-cryopreservation with a 1:9 milt-diluent ratio. The mean viability of T. soro spermatozoa post-cryopreservation in 0 %, 10 %, 20 %, and 30 % concentrations are 12.01 ? 2.24 %, 8.10 ? 4.53 %, 53 ? 5.14 %, and 6.23 ? 4 % respectively. The results of the one-way ANOVA test showed that the apple juice in various concentrations did not affect the viability of T. soro spermatozoa 24 hours post-cryopreservation (P>0.5).
KW - apple juice
KW - Malus sylvertris-Mill
KW - Tor soro
KW - xidant
UR - http://www.scopus.com/inward/record.url?scp=85117938774&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/846/1/012003
DO - 10.1088/1755-1315/846/1/012003
M3 - Conference article
AN - SCOPUS:85117938774
SN - 1755-1307
VL - 846
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012003
T2 - 4th Life and Environmental Sciences Academics Forum, LEAF 2020
Y2 - 6 November 2020 through 7 November 2020
ER -