@inproceedings{af47c2508c3043dba0015d46633e43e6,
title = "The effect of various temperatures of heat shock method to the DNA concentration of filamentous strains Cyanobacteria",
abstract = "Filamentous Cyanobacteria commonly has mucilaginous sheath as cells' protection from any physical damage, such as dehydration or UV-light. In many experiences, the presence of sheath in the process of DNA extraction caused low concentration and purity of DNA samples. In order to optimize DNA extraction for species identification, a method called heat shock was used in present study. In general, heat shock refers to cellular exposure to rapid stress changes such as temperature. Three strains of Cyanobacteria from genus Nostoc (SO-24), Cylindrospermum (SO-163), and Fischerella (SO-133) were used as samples based on their characteristic of mucilaginous sheath. Three variations of temperature were used in present study: 50, 80 and 100 °C. The DNA yield and purity for the method were evaluated using spectrophotometry and compared. The results showed that DNA extraction gained maximum DNA concentration at temperature of 50 °C. Temperature did not have a correlation with DNA purity of the samples. Each strain had different response to the method and this might be because the thickness of the sheath on each strains.",
keywords = "DNA extraction, extracellular polysaccharide, extraction technique",
author = "R. Lestari and Nasrul, {A. A.} and Pitaloka, {K. D.} and D. Hendrayanti",
note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",
year = "2019",
month = nov,
day = "4",
doi = "10.1063/1.5132529",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",
booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",
address = "United States",
}