The Effect of Soybean and Soybean Meal Extract on COX-2 and iNOS Expression in Colon Preneoplasia of Mice Induced by Azoxymethane and Dextran Sodium Sulfate

Several studies shows an increase in colon cancer case which is triggered by lifestyle changes in society such as smoking, obesity, high-fat diet, consumption of burned food and lack of fiber consumption. This study objective is to evaluate the inhibition activities of soybean seed extract and soybean meal extract on COX-2and iNOS expressions in colon preneoplasia of mice induced by azoxymethane and dextran sodium sulfate. Swiss Webster mice are injected intraperitoneally by single dose of 10 mg/kg BW azoxymethane and after 7 days followed by administration of 2% dextran sodium sulfatein their drinking for a week. Both extracts are administered orally in three different doses(75 mg / 20 gBW, 150 mg / 20 gBW and 200 mg / 20 gBW) daily for 4 weeks. Immunohistochemical examination is conducted to see the cells with the expression of COX 2 and iNOSin every 1000 epithelial cells. The result shows both the extracts decreases the expression of COX-2 at dose 150 mg / 20 g body weight and 200 mg / 20 g BW significantly with P