Research regarding Graphene Oxide (GO) based nanofluids was done in this present day because of its thermal conductivity. In this study, modified Hummers Method selected to synthesize GO. GO nanoparticle then characterized by EDS, SEM, and XRD. GO nanoparticle then dispersed in water as its base fluid with concentration of 0,01%, 0,03%, and 0,05%. Sodium Dodecyl Benzene Sulfonate (SDBS) surfactant was added with the concentration of 10% and 20% for a better stability. The mixing process is done by ultrasonication for 2 hours. Nanofluids then characterized by Particle Size Analyzer (PSA), zeta potential, and thermal conductivity. The PSA characterization showed the size of particle is more than 100nm so this fluid is still categorized as microparticles dispersed in fluid. Results showed that increasing of GO microparticle without surfactant at 0,01% to 0,03% enhanced the thermal conductivity of fluids, but at 0,05% the value was decreased with possibility of agglomeration. The increase of SDBS concentration at all fluids showed the decrease of thermal conductivity value. The property of GO microparticle which hydrophilic and anionic SDBS surfactant have a mutual charge which tend to make electrostatic repulsive force so the stability of the fluid and its heat transfer effectivity was decreased.