The effect of skim milk combined with 5% of methanol to preserve spermatozoa for 24 hours at-34°C has been studied. The objective of the study was to determine the optimum concentration of skim milk on preserving spermatozoa in sub-zero freezing. Skim milk used in this study were, 0%, 5%, 10%, 15%, 20%, and 25%, respectively. The sperms were diluted with the combination of 5% methanol, fish ringer and skim milk (1 part of sample + 5 part of solvent) and were equilibrated for 20 minutes at 4°C before freezed for 24 hours at-34°C. After freezed, sample (sperms) were thawed for 13 sec. at 40°C. Spermatozoa viability, motility, and abnormality, were then observed. The results showed that 5% of methanol combined with 20% of skim milk displayed the highest spermatozoa viability (81.75±8.22%), and the highest spermatozoa motility rate (83.23±3.27%). On the other hand, the combination of 5% methanol and 20% of skim milk showed the lowest abnormality (26.25±1.89%). It was concluded that 20% of skim milk combined with 5% of methanol was the optimum concentration for Java barb (Barbonymus gonionotus) spermatozoa cryopreservation compared to other skim milk concentration tested.
|Number of pages||8|
|Publication status||Published - 13 Apr 2016|
- Short-term preservation
- Spermatozoa quality
- Sub-zero freezing