TY - JOUR
T1 - The effect of skim milk combined with 5% of methanol on motiliy, viability, and abnormality of java barb, barbonymus gonionotus spermatozoa after 24 hours freezing
AU - Abinawanto, null
AU - Zuraida, Zuraida
AU - Lestari, Retno
N1 - Publisher Copyright:
© 2016, BIOFLUX SRL. All rights reserved.
PY - 2016/4/13
Y1 - 2016/4/13
N2 - The effect of skim milk combined with 5% of methanol to preserve spermatozoa for 24 hours at-34°C has been studied. The objective of the study was to determine the optimum concentration of skim milk on preserving spermatozoa in sub-zero freezing. Skim milk used in this study were, 0%, 5%, 10%, 15%, 20%, and 25%, respectively. The sperms were diluted with the combination of 5% methanol, fish ringer and skim milk (1 part of sample + 5 part of solvent) and were equilibrated for 20 minutes at 4°C before freezed for 24 hours at-34°C. After freezed, sample (sperms) were thawed for 13 sec. at 40°C. Spermatozoa viability, motility, and abnormality, were then observed. The results showed that 5% of methanol combined with 20% of skim milk displayed the highest spermatozoa viability (81.75±8.22%), and the highest spermatozoa motility rate (83.23±3.27%). On the other hand, the combination of 5% methanol and 20% of skim milk showed the lowest abnormality (26.25±1.89%). It was concluded that 20% of skim milk combined with 5% of methanol was the optimum concentration for Java barb (Barbonymus gonionotus) spermatozoa cryopreservation compared to other skim milk concentration tested.
AB - The effect of skim milk combined with 5% of methanol to preserve spermatozoa for 24 hours at-34°C has been studied. The objective of the study was to determine the optimum concentration of skim milk on preserving spermatozoa in sub-zero freezing. Skim milk used in this study were, 0%, 5%, 10%, 15%, 20%, and 25%, respectively. The sperms were diluted with the combination of 5% methanol, fish ringer and skim milk (1 part of sample + 5 part of solvent) and were equilibrated for 20 minutes at 4°C before freezed for 24 hours at-34°C. After freezed, sample (sperms) were thawed for 13 sec. at 40°C. Spermatozoa viability, motility, and abnormality, were then observed. The results showed that 5% of methanol combined with 20% of skim milk displayed the highest spermatozoa viability (81.75±8.22%), and the highest spermatozoa motility rate (83.23±3.27%). On the other hand, the combination of 5% methanol and 20% of skim milk showed the lowest abnormality (26.25±1.89%). It was concluded that 20% of skim milk combined with 5% of methanol was the optimum concentration for Java barb (Barbonymus gonionotus) spermatozoa cryopreservation compared to other skim milk concentration tested.
KW - Short-term preservation
KW - Spermatozoa quality
KW - Sub-zero freezing
UR - http://www.scopus.com/inward/record.url?scp=84964424244&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84964424244
SN - 1844-8143
VL - 9
SP - 326
EP - 333
JO - AACL Bioflux
JF - AACL Bioflux
IS - 2
ER -