The effect of omega-3-enriched fish oil on the inflammation of mice colon induced by AOM and DSS: Study on COX-2

Introduction: Colorectal cancer is one of the leading causes of cancer deaths in some countries of the world. Inflammation has been recognized as one of the etiologies of colorectal cancer. Omega-3 is known to have anti-inflammatory properties. The aim of this tudy was to investigate the anti-inflammatory effect of omega-3-enriched-fish oil on inflammation of mice’s colon by induction of azoxymethane (AOM) and dextran sodium sulfate (DSS) by analyzing the expression of cyclooxygenase-2 (COX-2). Method: 30 Swiss Webster mice divided into 6 groups: (1) Negative control group (KN); (2) test group of fish oil with a dose of 1.5 mg / day (Dose-I); (3) test group of fish oil with a dose of 3 mg / day (Dose-II); (4) solvent-control group of corn oil (KP); (5) Positive control group of aspirin (ASP); (6) Normal group (KNO). Group 1-5 was induced with AOM 10 mg / kgBB intraperitoneally once, then 1 week later followed by administering 2% ad lib DSS in drinking water for 7 days. After the induction period, continued treatment time based on group above for 6 weeks. Results: There were no significant diffenrence in the test group (Dosis-I dan Dosis-II) (p>0.05) related to COX-2 compared with all control groups. Significant differences in the expression of COX-2 were found by comparing all treatment groups with the normal group (p <0.05). Conclusion: omega-3-rich fish oils with dose of 3 mg and 1.5 mg were not able to inhibit inflammatory proces of AOM+DSS-induced colon mice.