The effect of nitrogen addition and homogenization in hydrolysis process on mucilaginous sheath removal of filamentous Cyanobacteria

A. A. Nasrul, D. Hendrayanti, K. D.P. Loka, S. W.R. Hilia, R. Lestari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Research on the hydrolysis technique to remove mucilaginous sheath of filamentous Cyanobacteria has been conducted. The research aims to know the effect of addition of nitrogen in medium and homogenization with glass beads on mucilaginous sheath removal of filamentous Cyanobacteria. Three strains of Cyanobacteria used in present study were Nostoc sp. SO-24, Cylindrospermum sp. SO-115, and Calothrix sp. SO-163. Each strain was grown in Blue-Green 11 (BG-11) medium with and without nitrogen content, then homogenized with 3.2 mm glass beads, and hydrolyzed. The result biomass was extracted to get the DNA. The result of hydrolysis process was examined through microscopic observation with and without negative staining. The result showed that addition of nitrogen and homogenization+hydrolysis process affected the mucilaginous sheath removal in all strains. Through this treatment, DNA concentration of SO-24 and SO-163 were higher than SO-115. Purity of DNA of all strains was low, ranging from 0.79-1.71.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Ammonium
  • cyanobacteria DNA
  • polysaccharide sheath

Fingerprint Dive into the research topics of 'The effect of nitrogen addition and homogenization in hydrolysis process on mucilaginous sheath removal of filamentous Cyanobacteria'. Together they form a unique fingerprint.

Cite this