The effect of mouthwash combination of immunoglobulin-y anti-comd streptococcus mutans and chitosan on the formation of streptococcus mutans biofilm

Endang Winiati Bachtiar, Deta Apritantia, Agoeng Tjahajani Sarwono, Boy M. Bachtiar, Retno D. Soejoedono, I. Wayan Teguh Wibawan

Research output: Contribution to journalArticle

Abstract

Objective: The objective of this study was to investigate the effectiveness of immunoglobulin Y (IgY) antibodies as a dental caries vaccine by utilizing IgY specific to the quorum-sensing signaling receptor ComD of Streptococcus mutans combined with chitosan in the form of mouthwash. Methods: The effects of a mouthwash containing IgY anti-ComD S. mutans with and without chitosan on biofilm-forming isolates of S. mutans were investigated. Subjects were assigned to rinsing twice daily for 6 days with 15–20 ml mouthwash solution for 30 s. Biofilm formation of S mutans isolated from the patiens was measured using a crystal violet method to determine the optical density at 490 nm. Results: The results indicated that mouthwash containing IgY anti-ComD S. mutans and chitosan significantly enhanced the biofilm formation of S. mutans. In mouthwash containing IgY anti-ComD S. mutans without chitosan, a reduction in biofilm formation was observed; however, this was not statistically significant. Conclusions: The mouthwash combination of IgY anti-ComD S. mutans and chitosan enhanced the biofilm formation ability of S. mutans isolated from caries and caries-free subjects. Further, research is needed to determine the appropriate concentrations of IgY anti-ComD S. mutans and chitosan required to effectively inhibit dental caries.

Original languageEnglish
Pages (from-to)110-113
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume11
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • Biofilm
  • Chitosan
  • IgY
  • Mouthwash
  • Streptococcus mutans

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