TY - JOUR
T1 - The effect of mesenchymal stem cell-enriched scaffolds on mmp-8 and tgf-β levels of vertebrae postlaminoplasty in rabbit model
AU - Rahyussalim, Ahmad Jabir
AU - Sahputra, Roni Eka
AU - Yanwirasti,
AU - Manjas, Menkher
AU - Whulanza, Yudan
AU - Kurniawati, Tri
AU - Aprilya, Dina
AU - Zufar, Muhammad Luqman Labib
N1 - Publisher Copyright:
© 2021 Rahyussalim et al.
PY - 2021
Y1 - 2021
N2 - Introduction: Some laminoplasty procedures still have restenosis because of bony-bridging failure of the laminar hinge. The present study aimed to determine the effect of mesenchymal stem cell (MSC)-enriched scaffolds on vertebral regeneration after laminoplasty on the basis of the number of osteoblasts, matrix metalloproteinase-8 (MMP-8), and transforming growth factor-beta (TGF-β) levels. Methods: Laminoplasty procedure using the Hirabayashi technique was conducted at the lumbar level in 32 rabbits that were divided into four and three groups of the control (C) and treatment groups, respectively, with different types of laminoplasty spacer (T1, autograft; T2, scaffold; and T3, scaffold with MSCs). Histopathological studies were conducted to calculate the number of osteoblasts and enzyme-linked immunosorbent assay tests to detect MMP-8 and TGF-β 4 weeks after the surgery. Results: The results showed a significant decrease in MMP-8 level in the T3 group compared with that in the control group (p < 0.05). A significant difference exists between the average number of newly formed osteoblasts in the control group compared with that in the T3 group (p < 0.05) with a higher mean blood TGF-β level of all experimental groups compared with that of the control group (p = 0.58). Conclusion: The significant decrease in MMP-8 levels, increase in TGF-β levels, and increased number of osteoblasts on MSC-seeded polylactic acid scaffolds could be useful to support the laminoplasty procedure to prevent restenosis because it was biocompatible and promoted the bone healing process.
AB - Introduction: Some laminoplasty procedures still have restenosis because of bony-bridging failure of the laminar hinge. The present study aimed to determine the effect of mesenchymal stem cell (MSC)-enriched scaffolds on vertebral regeneration after laminoplasty on the basis of the number of osteoblasts, matrix metalloproteinase-8 (MMP-8), and transforming growth factor-beta (TGF-β) levels. Methods: Laminoplasty procedure using the Hirabayashi technique was conducted at the lumbar level in 32 rabbits that were divided into four and three groups of the control (C) and treatment groups, respectively, with different types of laminoplasty spacer (T1, autograft; T2, scaffold; and T3, scaffold with MSCs). Histopathological studies were conducted to calculate the number of osteoblasts and enzyme-linked immunosorbent assay tests to detect MMP-8 and TGF-β 4 weeks after the surgery. Results: The results showed a significant decrease in MMP-8 level in the T3 group compared with that in the control group (p < 0.05). A significant difference exists between the average number of newly formed osteoblasts in the control group compared with that in the T3 group (p < 0.05) with a higher mean blood TGF-β level of all experimental groups compared with that of the control group (p = 0.58). Conclusion: The significant decrease in MMP-8 levels, increase in TGF-β levels, and increased number of osteoblasts on MSC-seeded polylactic acid scaffolds could be useful to support the laminoplasty procedure to prevent restenosis because it was biocompatible and promoted the bone healing process.
KW - Hirabayashi technique
KW - Laminoplasty
KW - Mesenchymal stem cells
KW - Scaffolds
UR - http://www.scopus.com/inward/record.url?scp=85111052490&partnerID=8YFLogxK
U2 - 10.2147/SCCAA.S314107
DO - 10.2147/SCCAA.S314107
M3 - Article
AN - SCOPUS:85111052490
SN - 1178-6957
VL - 14
SP - 27
EP - 37
JO - Stem Cells and Cloning: Advances and Applications
JF - Stem Cells and Cloning: Advances and Applications
ER -