TY - JOUR
T1 - The effect of Mahkota Dewa (Phaleria Macrocarpa) (Scheff.) fruit pericarp extract on COX-2 and β-catenin expression in mice colon induced by dextran sodium sulfate
AU - Kusmardi, null
AU - Estuningtyas, Ari
AU - Shavera, D.
AU - Tedjo, Aryo
AU - Priosoeryanto, B. P.
AU - Habiburrahman, M.
AU - Salinah,
N1 - Funding Information:
We would like to thank for Directorate of Research and Public Services Universitas Indonesia for PITTA 2017 Grand and Ministry of Research, Technology, and Higher Education for PUPT 2016 grand.
Publisher Copyright:
© 2018, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.
PY - 2018/8/30
Y1 - 2018/8/30
N2 - The objective of this research was to investigate the anti-inflammatory effect of mahkota dewa (Phaleria macrocarpa) fruit pericarp extract on COX-2 and β-catenin expression in mice colon induced by dextran sodium sulphate (DSS). The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of COX-2 and β-catenin expression by immunohistochemistry assay at four different doses, i.e. 650 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg. Swiss webster male mice weighing 25-30 g were used for the study. The results of COX-2 optical density score in dose 625 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg were 1.58, 1.52, 1.47, 1.33, respectively. This result was significantly different (p = 0.000) with DSS group that was 2.46 Meanwhile β-catenin optical density score in dose 625 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg were 1.46, 1.37, 1.41, 1.36, respectively. This result was significantly different (p = 0.000) with DSS group that was 2.34. Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in experimental model shown by suppressing expression of COX-2 and β-catenin.
AB - The objective of this research was to investigate the anti-inflammatory effect of mahkota dewa (Phaleria macrocarpa) fruit pericarp extract on COX-2 and β-catenin expression in mice colon induced by dextran sodium sulphate (DSS). The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of COX-2 and β-catenin expression by immunohistochemistry assay at four different doses, i.e. 650 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg. Swiss webster male mice weighing 25-30 g were used for the study. The results of COX-2 optical density score in dose 625 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg were 1.58, 1.52, 1.47, 1.33, respectively. This result was significantly different (p = 0.000) with DSS group that was 2.46 Meanwhile β-catenin optical density score in dose 625 mg/kg, 1250 mg/kg, 2500 mg/kg and 5000 mg/kg were 1.46, 1.37, 1.41, 1.36, respectively. This result was significantly different (p = 0.000) with DSS group that was 2.34. Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in experimental model shown by suppressing expression of COX-2 and β-catenin.
KW - Colon
KW - Cyxlooxygenase-2
KW - Dextran sodium sulphate
KW - Pericarp
KW - Phaleria macrocarpa
KW - β-catenin
UR - http://www.scopus.com/inward/record.url?scp=85052757247&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85052757247
VL - 2
SP - 199
EP - 205
JO - Pharmacologyonline
JF - Pharmacologyonline
SN - 1827-8620
ER -