TY - JOUR
T1 - The effect of mahkota dewa (Phaleria macrocarpa) (scheff.) fruit pericarp extract on inos in mice colon intermittently-induced by dextran sodium sulfate
AU - Kusmardi, K.
AU - Estuningtyas, Ari
AU - Shavera, Dilla
AU - Tedjo, Aryo
AU - Priosoeryanto, B. P.
N1 - Funding Information:
We would like to thank for Directorate of Research and Public Services Universitas Indonesia, for PITTA 2017 Grand and Ministry of Research, Technology, and Higher Education for PUPT 2016 Grand.
Publisher Copyright:
© 2017 The Authors.
PY - 2017
Y1 - 2017
N2 - Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS). Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The antiinflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study. Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24. Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS.
AB - Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS). Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The antiinflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study. Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24. Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS.
KW - Colon
KW - Dextran sodium sulfate
KW - Inducible nitric oxide synthase
KW - Inflammatory cells
KW - Pericarp
KW - Phaleria macrocarpa
UR - http://www.scopus.com/inward/record.url?scp=85036665176&partnerID=8YFLogxK
U2 - 10.22159/ajpcr.2017.v10i12.20560
DO - 10.22159/ajpcr.2017.v10i12.20560
M3 - Article
AN - SCOPUS:85036665176
VL - 10
SP - 309
EP - 312
JO - Asian Journal of Pharmaceutical and Clinical Research
JF - Asian Journal of Pharmaceutical and Clinical Research
SN - 0974-2441
IS - 12
ER -