The effect of magnesium on halothane-induced intracellular calcium concentration changes in cardiomyocytes

Soenarto Ratna Farida, S. Madjid Amir, D. Suyatna Frans, R. Fera Ibrahim, Basuki Endang, Nurhadi Ibrahim

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Halothane, a potent inhalational anesthetic, is known to cause arrhythmia, probably due to Ryanodine Receptor (RyR) activation, triggering Ca 2+ release from sarcoplasmic reticulum (SR) to the cytosol. Mg 2+ hypothetically prevents Ca 2+ release by inhibiting RyR and increasing Ca 2+ reuptake to SR. Methods: An in vitro experiment was done on cultured cell of rat cardiomyocytes. Cells were divided into six groups. Five groups were exposed to 2 mM halothane (1–3 MAC) for 5 minutes and one was not. Of the 5 halothane-exposed groups, group 1 received no additional treatment and was observed at minute-0, 5, 10, 15 and 20 after discontinuation. Group 2 and 3 received 11 mM and 22 mM MgSO 4 after halothane exposure, respectively. Group 4 and 5 received corresponding MgSO 4 treatment before exposure. Cytosolic Ca 2+ change was observed by a confocal microscope and emission was measured by pixel analysis. Results: Halothane increased cytosolic Ca 2+ concentration in rat cardiomyocytes, in which was not substantially altered by MgSO 4 given before or after the exposure. Higher dose of Mg 2+ resulted in decreasing Ca 2+ concentration. 22 mM MgSO 4 decreased cytosolic Ca 2+ concentration to the same extent as halothane discontinuation for 10 minutes. Fifteen minutes halothane discontinuation significantly decreased cytosolic Ca 2+ concentration and 20 minutes discontinuation returned the Ca 2+ concentration to the basal level. Conclusion: Halothane increases cytosolic Ca 2+ concentration in rat cardiac myocytes. Neither pre-nor post-halothane exposure administration of MgSO 4 substantially alters this phenomenon. Discontinuation of halothane for 15 minutes significantly reduces cytosolic Ca 2+ concentration.

Original languageEnglish
Pages (from-to)6803-6806
Number of pages4
JournalAdvanced Science Letters
Volume23
Issue number7
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • Cardiac myocyte
  • Cytosolic Ca
  • Halothane
  • Mg

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