The effect of magnesium on halothane-induced intracellular calcium concentration changes in cardiomyocytes

Soenarto Ratna Farida, S. Madjid Amir, D. Suyatna Frans, R. Fera Ibrahim, Basuki Endang, Nurhadi Ibrahim

Research output: Contribution to journalArticlepeer-review


Background: Halothane, a potent inhalational anesthetic, is known to cause arrhythmia, probably due to Ryanodine Receptor (RyR) activation, triggering Ca2+release from sarcoplasmic reticulum (SR) to the cytosol. Mg2+hypothetically prevents Ca2+release by inhibiting RyR and increasing Ca2+reuptake to SR. Methods: An in vitro experiment was done on cultured cell of rat cardiomyocytes. Cells were divided into six groups. Five groups were exposed to 2 mM halothane (1–3 MAC) for 5 minutes and one was not. Of the 5 halothane-exposed groups, group 1 received no additional treatment and was observed at minute-0, 5, 10, 15 and 20 after discontinuation. Group 2 and 3 received 11 mM and 22 mM MgSO4after halothane exposure, respectively. Group 4 and 5 received corresponding MgSO4treatment before exposure. Cytosolic Ca2+change was observed by a confocal microscope and emission was measured by pixel analysis. Results: Halothane increased cytosolic Ca2+concentration in rat cardiomyocytes, in which was not substantially altered by MgSO4given before or after the exposure. Higher dose of Mg2+resulted in decreasing Ca2+concentration. 22 mM MgSO4decreased cytosolic Ca2+concentration to the same extent as halothane discontinuation for 10 minutes. Fifteen minutes halothane discontinuation significantly decreased cytosolic Ca2+concentration and 20 minutes discontinuation returned the Ca2+concentration to the basal level. Conclusion: Halothane increases cytosolic Ca2+concentration in rat cardiac myocytes. Neither pre-nor post-halothane exposure administration of MgSO4substantially alters this phenomenon. Discontinuation of halothane for 15 minutes significantly reduces cytosolic Ca2+concentration.

Original languageEnglish
Pages (from-to)6803-6806
Number of pages4
JournalAdvanced Science Letters
Issue number7
Publication statusPublished - Jul 2017


  • Cardiac myocyte
  • Cytosolic Ca
  • Halothane
  • Mg


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