BACKGROUND: Involving natural food ingredients in controlling blood glucose in diabetic patients has been widely studied. However, there is little information about the effect of the ingredients on improvement of clinical, laboratory, and gut function markers. OBJECTIVE: This study was aimed to examine the effect of Job's tears-enriched yoghurt on weight, fasting blood glucose, GLP-1, and Calprotectin in patients with type 2 Diabetes Mellitus. METHODS: Sixty male and female subjects with T2DM participating in this study were divided in two groups; received yoghurt (control group/C) and Job's tears-enriched yoghurt (treatment group/T). Subjects use metformin only were allowed. Job's tears seed was extracted using a standard process. Height and weight were measured. Laboratory examination for fasting blood glucose, GLP-1, and calprotectin were performed using standard methods. Appropiate statistical analysis tests were applied to examine variables change within and between groups. Significancy was set at p <0.05.