The effect of higher temperature on the aggressiveness of the mice adenocarcinoma mammae cells

The incidence of breast cancer patients was considered high both in the world and in Indonesia. The pathogenesis of breast cancer is influenced by many factors and one of the factors that would be focus on in this research is temperature. The aim of this research is to analyze the temperature effect on the proliferation of adenocarcinoma mammae cells in vivo. A true experimental design is used with C3H mice exposed to: 20-22°C, 25-27°C, and 32-34°C for 6 hours daily in 2 weeks. Two kinds of staining were used, agyrophilic nucleolar organiser region (AgNOR) for cells proliferation and Hematoxylin-Eosin (HE) for mitotic count. AgNORs result revealed that the mean AgNORs (mAgNORs) and proliferative index AgNORs (pAgNORs) of group 32-34°C was the highest and the differences are significant with p= 0,000 through one-way ANOVA. HE result showed that there was a tendency of group 32-34°C to have a higher mitotic count compared to the other two groups but was insignificant through Mann-Whitney. The result of the AgNORs study is expected to give an idea for breast cancer non-invasive therapy by manipulating environmental temperature.