The effect of grape seed extraction irrigation solution towards cleanliness the smear layer on apical third of the root canal wall

Grape seed Extract (GSE) contains 74-78% proantosianidin that serves as an accelerator of crosslinking collagen. The aim of this study was to analyze the ability of GSE as root canal irrigation solution in cleaning smear layer on the apical third area. Fifty extracted incisors teeth were divided into 5 groups. As an irrigation solution; group 1 were using distilled water (aquadest), group 2: 3.25% GSE, group 3: 6.5% GSE, group 4: 13% GSE and group 5: 17% EDTA. The cleanliness of smear layer were evaluated by SEM and then scored. The data were analyzed by using Kolmogorov-Smirnov by SPSS 17. The highest score is on the 13% GSE group (40%) followed by 17% EDTA and 3.25% GSE (30%), while in group of aquadest was 0%. Score 2 showed that aquadest was the lowest level of cleanliness around 80%, followed by 13% GSE group, 3.25% GSE and 6.5% GSE. Whereas in 17% EDTA group there was no score 2. The whole group of GSE compared with aquadest and EDTA showed no significant differences amongst them Grape seed extract solution has a potency for cleaning the smear layer on apical third area of the root canal.