The effect of fermentation temperature and the crude extract concentration of bitter leaf (Andrographis Paniculata) on inhibition of α-glucosidase enzyme activity for antidiabetic drugs

Ajrina Adistiabudi Khairani, Sarah Fairuz Imani, Rita Arbianti, Tania Surya Utami, Heri Hermansyah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Diabetes mellitus (DM) is a metabolic disease characterized by an increase in blood glucose level above normal (hyperglycemia). Natural ingredients containing hypoglycemic substances can be used an alternative DM treatment, one of which is bitter leaf (Andrographis paniculata). Sambiloto leaves contain compositions of andrographolide and flavonoids which have anti-diabetic activity. The research method begins with simplicia preparation which begins with fermentation using Aspergillus niger with a fermentation temperature variation of 26°C, 30°C, 34°C, and 37°C. The sample is then extracted with a frequency of 42 kHz with 70% ethanol. The extraction results were used in the anti-diabetes test by comparing samples given α-glucosidase enzymes with positive control (diabetes medication) and negative control (ethanol 70% with corresponding concentration), and samples without fermentation process with coarse variations of crude extract 100 ppm, 200 ppm, 300 ppm, 500 ppm, and 5 mg/ml and added absorbance using UV-Vis spectrophotometry. Chemical components analysis was carried out using GC-MS.

Original languageEnglish
Title of host publication4th International Tropical Renewable Energy Conference, i-TREC 2019
EditorsEny Kusrini, I. Gde Dharma Nugraha
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420144
DOIs
Publication statusPublished - 3 Sep 2020
Event4th International Tropical Renewable Energy Conference 2019, i-TREC 2019 - Bali, Indonesia
Duration: 14 Aug 201916 Aug 2019

Publication series

NameAIP Conference Proceedings
Volume2255
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Tropical Renewable Energy Conference 2019, i-TREC 2019
Country/TerritoryIndonesia
CityBali
Period14/08/1916/08/19

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