TY - JOUR
T1 - The Effect of Fasting on Oxidative Stress in the Vital Organs of New Zealand White Rabbit
AU - Hardiany, Novi Silvia
AU - Karman, Adrian Prasetya
AU - Calista, Aisha Safa Putri
AU - Anindyanari, Budi Gittanaya
AU - Rahardjo, Daniell Edward
AU - Novira, Puti Retasya
AU - Taufiq, Rania Rifdah
AU - Imtiyaz, Shofiyya
AU - Antarianto, Radiana Dhewayani
N1 - Funding Information:
The authors thank to Direktorat Riset dan Pengabdian Masyarakat (DRPM) Universitas Indonesia for research grant Publikasi Terindeks Internasional/PUTI Q3 2020 (Grant number: NKB-1874/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2022
PY - 2022/7
Y1 - 2022/7
N2 - Background: Oxidative stress is defined as the condition in which balance between the synthesis and detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand White (NZW) rabbits. Methods: Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting (PF) groups. The controls were fed ad lib. IF and PF groups were fasted for 16 and 40 hours, respectively, followed by eight hours of non-fasting, for six days and were sacrificed on the 7th day. One hundred mg of heart, brain, and kidney tissues were homogenized in 1 ml of phosphate-buffered saline. MDA, carbonyl, GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and post hoc test. Results: In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was significantly less in the PF than in the control group. Conclusions: The effect of fasting on oxidative stress in various organs showed various responses, however fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.
AB - Background: Oxidative stress is defined as the condition in which balance between the synthesis and detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand White (NZW) rabbits. Methods: Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting (PF) groups. The controls were fed ad lib. IF and PF groups were fasted for 16 and 40 hours, respectively, followed by eight hours of non-fasting, for six days and were sacrificed on the 7th day. One hundred mg of heart, brain, and kidney tissues were homogenized in 1 ml of phosphate-buffered saline. MDA, carbonyl, GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and post hoc test. Results: In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was significantly less in the PF than in the control group. Conclusions: The effect of fasting on oxidative stress in various organs showed various responses, however fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.
KW - Fasting
KW - Oxidative stress
KW - Rabbit
KW - Vital organs
UR - http://www.scopus.com/inward/record.url?scp=85134709747&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85134709747
SN - 2322-3480
VL - 11
SP - 190
EP - 199
JO - Reports of Biochemistry and Molecular Biology
JF - Reports of Biochemistry and Molecular Biology
IS - 2
ER -