The effect of ethanolic leaves extract of soursop (Annona muricata L.) on human colorectal cancer cell line: cell viability and in silico study to cyclin D1 protein

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Colorectal cancer is a pathological transformation of normal colon and rectum epithelial
that becomes an abnormal tissue mass, due to the overexpression of cyclin D1 protein that inducing the
high proliferation of colorectal cell. Treatment and prevention of colorectal cancer could be done
naturally by consuming leave extract of Annona muricata L. (soursop). Soursop is known for many
phytochemical components such as alkaloid, annonaceous acetogenin, phenol, and flavonol that serve as
an anti-cancer.
Method: The research was used HT-29 colorectal cell that given ethanolic leave extract of soursop with
278 μg/mL concentration, 5-Fluorourasil (5-FU) with 88 μg/mL concentration as positive control, solvent
control, dan cells control as negative control. The parameters are cell viability with MTT Assay and
analysis of molecular docking from ethanolic leave extract of soursop to cyclin D1 protein with
molecular operating environment (MOE) 2013.08 software.
Result: Percentage of viable HT-29 cell line decrease in accordance with increasing concentration and
the lowest percentage of viable cell is 2 x cytotoxicity concentration 50 (CC50) after ethanolic leave
extract of soursop treatment (40,4±1,3%) was compared to 5-FU (30,68±0,93%), solvent control
(97,2±1,4%), and cells control (100%). Analysis of molecular docking to cyclin D1 protein was obtained
N-hexadecanoic acid and phytol molecules that have the lowest free energy (ΔG), i.e 9,7755 kkal/mol
and -7,2147 kkal/mol.
Conclusion: Ethanolic leave extract of soursop causes decreasing cell viability of HT-29 cell line on 2 x
CC50 concentration was compared to 5-FU, solvent control, dan cells control. N-hexadecanoic acid and
phytol molecules have ability to inhibit cyclin D1 protein.
Original languageEnglish
JournalHealth Science Journal of Indonesia
Publication statusPublished - 2020

Fingerprint

Dive into the research topics of 'The effect of ethanolic leaves extract of soursop (Annona muricata L.) on human colorectal cancer cell line: cell viability and in silico study to cyclin D1 protein'. Together they form a unique fingerprint.

Cite this