The effect of environmental temperature on the mammary cancer cells and the infiltrating lymphocytes

Kusmardi Kusmardi, Yuli Felistia, Puspita Eka Wuyung, R. I.A. Kodariah, Dextra F. Dzakirosin

Research output: Contribution to journalArticle

Abstract

Purpose: To identify whether temperature has effect on proliferation in the tumor cell (adenocarcinoma) especially in vivo condition Methodology: The experiment was conducted in true experimental design (parallel), in which C3H mice are used, arranged in three cages with different temperatures: 20-22°C, 25-27°C, and 32-34°C in 2 weeks every day for 6 hours each day. The proliferation of the cells was detected with Argyrophilic Nucleolar Organizer Region (AgNOR) staining (mAgNOR and pAgNOR) and lymphocytes were stained with Hematoxylin-Eosin staining. Results: As the result, One-Way ANOVA analysis shows p = 0,000 for mAgNOR and p = 0.000 for pAgNOR. In addition, Mann-Whitney analysis for lymphocyte counts shows p = 0.0241 for both 20-22°C & 25-27°C and 25-27°C & 32-34°C Applications/Originality/Value: The temperature affects cell proliferation in vivo. and number of tumor ilfiltrating lymphocytes.

Original languageEnglish
Pages (from-to)632-641
Number of pages10
JournalInternational Journal of Pharmaceutical Research
Volume11
Publication statusPublished - 1 Oct 2019

Keywords

  • AgNOR

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