The effect of egg yolk as natural cryoprotectant on giant grouper (Epinephelus lanceolatus) spermatozoa motility

T. P. Pratiwi, A. Abinawanto, R. Lestari, A. Bowolaksono, N. G. Zavitri

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This research was to determine the optimum concentration of egg yolk on spermatozoa motility of Giant Grouper 48 hours after cryopreservation. Egg yolk used in this study were 0 %, 5 %, 10 %, 15 %, 20 % and 25 %, respectively, combined with 6 % glycerol. Sperm was collected by a hand stripping method. Sperms were then conditioned at 4 °C for 10 minutes and were refrigerated at -20 °C for 48 hours. Thawing was carried out at 4 °C for 30-60 seconds. Spermatozoa motility was evaluated before and after frozen. According to Anova test, there was a notable impact (p≤0.05) from a varied contents of egg yolk on post-thaw spermatozoa motility. Based on the Tukey test, the concentration of 15 % of egg yolk showed the highest post-thaw spermatozoa motility (83.64±1.72 %) on post-thaw motility (p<0.05). Fifteen percent of egg yolk demonstrated the optimum concentration in this study.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Cryopreservation
  • egg yolk
  • giant grouper
  • sperm motility

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