The effect of curcumin on the proliferation and extracellular matrix production in ethanol-induced hepatic stellate cells

Rahmaniah Rahmaniah, Melva Louisa, Bantari Wk Wardhani, Vivian Soetikno

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Objectives: In various liver disease models, including those for alcoholic liver diseases, curcumin, a polyphenolic compound derived from Curcuma longa, is known to have an hepatoprotective effect. However, the mechanism of action underlying its effects on alcohol-induced hepatic fibrosis remains unknown. We aimed to investigate the mechanisms of action underlying the effects of curcumin, mainly involving the transforming growth factor (TGF)-β/Smad pathway. Methods: Hepatic stellate cells (HSCs), LX2, were incubated with 50 mM ethanol with or without curcumin (1 and 10 μM). Viable HSCs were counted using a LUNATM automated cell counter, whereas the expressions of TGF-β, Smad3, tissue inhibitor of metalloproteinases-1 (TIMP-1), and type 1 collagen mRNA were measured using quantitative reverse transcriptase polymerase chain reactions. Results: Curcumin significantly suppressed ethanol-induced HSCs proliferation. The antiproliferative effect of curcumin appeared to be dose dependent. In addition, the mRNA expressions of TGF-β, Smad3, TIMP-1, and type 1 collagen decreased in the cells treated with curcumin. Conclusion: Curcumin seems to attenuate ethanol-induced HSCs proliferation through the suppression of TGF-β and appears to reduce the production of extracellular matrix as shown by the decreased expression of type 1 collagen.

Original languageEnglish
Pages (from-to)168-171
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume11
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • Curcumin
  • Smad
  • Tissue inhibitor of metalloproteinases-1
  • Transforming growth factor-β
  • Type 1 collagen

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