The effect of Curcuma xanthorrhiza - Ethanol extract to biofilm formation of Streptococcus mutans and Aggregatibacter actinomycetemcomitans (Dental biofilm research: In vitro study)

Objective: This in vitro study aimed to analyze the mass ratio of single- and dual-species Streptococcus mutans and Aggregatibacter actinomycetemcomitans biofilm after exposure to Curcuma xanthorrhiza ethanol extract (Xan). Methods: A bacterial suspension in brain heart infusion medium, enriched with 0.2% sucrose, was exposed to the Xan, incubated for 18 hrs, and analyzed using a crystal violet assay. Results: This research concluded that the minimum inhibitory concentration of ethanol-temulawak extract against S. mutans was 5%, while the minimum bactericidal concentration was 15%. Conclusions: Xan prevented biofilm formation of single-species S. mutans and dual-species S. mutans and A. actinomycetemcomitans more effectively than it did single-species A. actinomycetemcomitans.