The effect of coconut milk in various concentrations on spermatozoa motility of Koi fish (Cyprinus carpio, Linnaeus 1758) 48 hours post-cryopreservation

Research about the effect of various concentrations of coconut milk on spermatozoa quality of Koi Fish (Cyprinus carpio, Linnaeus 1758) 48 hours post cryopreservation has been carried out. The research aims to understand the effect of 5 % methanol and various concentrations of coconut milk (0 %, 2 %, 4 %, 6 %, 8 % and 10 %) on the motility of Koi fish spermatozoa 48 hours post-cryopreservation. Koi fish milt in this research was collected by stripping method and then evaluated microscopically and macroscopically. Results obtained are further assayed statistically using the Saphiro-Wilk normality test, Levene homogeneity test, one way Anova test, and Tukey multiple comparison test. One way Anova test showed that various concentration of coconut milk had significant difference (P < 0.05) of average value of motility on spermatozoa of Koi fish 48 hours post cryopreservation. A combination of 5 % methanol with 6 % coconut milk is the optimum concentration of cryoprotectant because it produced the highest average value of spermatozoa motility (79.13 ± 4.18 %).