Previous research suggested that centrifugation is not necessary to see proteins <30 kDa. There is no standard procedure for regulating the centrifugation speed to separate proteins <30 kDa. The aim of this study is too determine the effect of centrifugation speeds of 11,000 g and 13,000 g on separating salivary supernatant proteins <30 kDa. After being centrifuged at 11,000 g and 13,000 g, the salivary protein profile was tested using SDS-PAGE. The frequency of salivary supernatant protein occurrence (after being centrifuged at 11,000 g and 13,000 g) were 35 and 45 with ranges of 9-27 kDa and 8-18 kDa respectively. Compared to 11,000 g, the centrifugation speed of 13,000 g separates more proteins <30 kDa with a narrower range.
|Journal||Journal of Physics: Conference Series|
|Publication status||Published - 30 Aug 2017|
|Event||1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017 - Depok, West Java, Indonesia|
Duration: 15 Jul 2017 → 16 Jul 2017