TY - JOUR
T1 - The effect of centrifugation speeds of 11,000 g and 13,000 g on small salivary protein profiles (less than 30 kDa)
AU - Nugraha, R. A.
AU - Baskoro, B. D.
AU - Puspitawati, Ria
AU - Redjeki, S.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2017/8/30
Y1 - 2017/8/30
N2 - Previous research suggested that centrifugation is not necessary to see proteins <30 kDa. There is no standard procedure for regulating the centrifugation speed to separate proteins <30 kDa. The aim of this study is too determine the effect of centrifugation speeds of 11,000 g and 13,000 g on separating salivary supernatant proteins <30 kDa. After being centrifuged at 11,000 g and 13,000 g, the salivary protein profile was tested using SDS-PAGE. The frequency of salivary supernatant protein occurrence (after being centrifuged at 11,000 g and 13,000 g) were 35 and 45 with ranges of 9-27 kDa and 8-18 kDa respectively. Compared to 11,000 g, the centrifugation speed of 13,000 g separates more proteins <30 kDa with a narrower range.
AB - Previous research suggested that centrifugation is not necessary to see proteins <30 kDa. There is no standard procedure for regulating the centrifugation speed to separate proteins <30 kDa. The aim of this study is too determine the effect of centrifugation speeds of 11,000 g and 13,000 g on separating salivary supernatant proteins <30 kDa. After being centrifuged at 11,000 g and 13,000 g, the salivary protein profile was tested using SDS-PAGE. The frequency of salivary supernatant protein occurrence (after being centrifuged at 11,000 g and 13,000 g) were 35 and 45 with ranges of 9-27 kDa and 8-18 kDa respectively. Compared to 11,000 g, the centrifugation speed of 13,000 g separates more proteins <30 kDa with a narrower range.
UR - http://www.scopus.com/inward/record.url?scp=85029597789&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/884/1/012062
DO - 10.1088/1742-6596/884/1/012062
M3 - Conference article
AN - SCOPUS:85029597789
SN - 1742-6588
VL - 884
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012062
T2 - 1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017
Y2 - 15 July 2017 through 16 July 2017
ER -