The effect of centrifugation speeds of 11,000 g and 13,000 g on small salivary protein profiles (less than 30 kDa)

R. A. Nugraha, B. D. Baskoro, Ria Puspitawati, S. Redjeki

Research output: Contribution to journalConference articlepeer-review

Abstract

Previous research suggested that centrifugation is not necessary to see proteins <30 kDa. There is no standard procedure for regulating the centrifugation speed to separate proteins <30 kDa. The aim of this study is too determine the effect of centrifugation speeds of 11,000 g and 13,000 g on separating salivary supernatant proteins <30 kDa. After being centrifuged at 11,000 g and 13,000 g, the salivary protein profile was tested using SDS-PAGE. The frequency of salivary supernatant protein occurrence (after being centrifuged at 11,000 g and 13,000 g) were 35 and 45 with ranges of 9-27 kDa and 8-18 kDa respectively. Compared to 11,000 g, the centrifugation speed of 13,000 g separates more proteins <30 kDa with a narrower range.

Original languageEnglish
Article number012062
JournalJournal of Physics: Conference Series
Volume884
Issue number1
DOIs
Publication statusPublished - 30 Aug 2017
Event1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017 - Depok, West Java, Indonesia
Duration: 15 Jul 201716 Jul 2017

Fingerprint Dive into the research topics of 'The effect of centrifugation speeds of 11,000 g and 13,000 g on small salivary protein profiles (less than 30 kDa)'. Together they form a unique fingerprint.

Cite this