The effect of centrifugation speed and Chitosan-Sodium Tripolyphosphate ratio toward the nanoencapsulation of Sambiloto (Andrographis paniculata) for the formulation of Hepatitis B drug

Hepatits B is a viral infection which attack the liver. One of the compound that can overcome and inhibits Hepatitis B is Andrographolide. The compound was derived from Sambiloto plants (Andrographis paniculate). Andrographolide compound works by inhibiting α-glucosidase which assists the secretion of Hepatitis B virus. The goal of this research is to make nanoencapsulation of sambiloto leaf extracts that was encapsulated in chitosan and STPP. The nanoencapsulation will increase the bioavailability of the body for the administered Andrographolide. The size of the resulting particle at a variation of centrifugal speed of 8.000 RPM with the concentration ratio of chitosan : STPP equals to 0.2%:0.1% (g/mL), was 68.3nm. The loading capacity of the nanoparticles is 67.20% and the encapsulation efficiency of the nanoparticles is 99.48%. The release profile has a cumulative release of 34.55% with slow release in gastric pH conditions and followed by a burst release in intestine pH conditions.