The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats

Muhammad Faris Rizqhilmi; Muhammad Ikrar Hermanadi; Muhammad Yusuf; Rani Wardani Hakim; Siti Farida; Erni Hernawati Purwaningsih

doi:10.1063/5.0100545

The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats

Muhammad Faris Rizqhilmi
, Muhammad Ikrar Hermanadi
, Muhammad Yusuf
, Rani Wardani Hakim
, Siti Farida
, Erni Hernawati Purwaningsih

Department of Pharmacy Pre Clinic

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.

Original language	English
Title of host publication	6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021
Editors	Siti Fauziyah Rahman, Ahmad Zakiyuddin, Yudan Whulanza, Nurul Intan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735443716
DOIs	https://doi.org/10.1063/5.0100545
Publication status	Published - 16 Aug 2022
Event	6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 - Depok, Virtual, Indonesia Duration: 7 Jul 2021 → 8 Jul 2021

Publication series

Name	AIP Conference Proceedings
Volume	2537
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Country/Territory	Indonesia
City	Depok, Virtual
Period	7/07/21 → 8/07/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

aging
Centella asiatica
heart
interleukin-10

Access to Document

10.1063/5.0100545

Cite this

Rizqhilmi, M. F., Hermanadi, M. I., Yusuf, M., Hakim, R. W., Farida, S., & Purwaningsih, E. H. (2022). The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats. In S. F. Rahman, A. Zakiyuddin, Y. Whulanza, & N. Intan (Eds.), 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021 Article 040008 (AIP Conference Proceedings; Vol. 2537). American Institute of Physics Inc.. https://doi.org/10.1063/5.0100545

Rizqhilmi, Muhammad Faris ; Hermanadi, Muhammad Ikrar ; Yusuf, Muhammad et al. / The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats. 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. editor / Siti Fauziyah Rahman ; Ahmad Zakiyuddin ; Yudan Whulanza ; Nurul Intan. American Institute of Physics Inc., 2022. (AIP Conference Proceedings).

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title = "The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats",

abstract = "The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.",

keywords = "aging, Centella asiatica, heart, interleukin-10",

author = "Rizqhilmi, \{Muhammad Faris\} and Hermanadi, \{Muhammad Ikrar\} and Muhammad Yusuf and Hakim, \{Rani Wardani\} and Siti Farida and Purwaningsih, \{Erni Hernawati\}",

note = "Funding Information: The researcher would like to thank the Health Research and Development Agency, Ministry of Health of the Republic of Indonesia, the Department of Pharmacy, and the Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, as well as all parties involved in this study. Publisher Copyright: {\textcopyright} 2022 American Institute of Physics Inc.. All rights reserved.; 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 ; Conference date: 07-07-2021 Through 08-07-2021",

year = "2022",

month = aug,

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language = "English",

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publisher = "American Institute of Physics Inc.",

editor = "Rahman, \{Siti Fauziyah\} and Ahmad Zakiyuddin and Yudan Whulanza and Nurul Intan",

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Rizqhilmi, MF, Hermanadi, MI, Yusuf, M, Hakim, RW , Farida, S & Purwaningsih, EH 2022, The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats. in SF Rahman, A Zakiyuddin, Y Whulanza & N Intan (eds), 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021., 040008, AIP Conference Proceedings, vol. 2537, American Institute of Physics Inc., 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021, Depok, Virtual, Indonesia, 7/07/21. https://doi.org/10.1063/5.0100545

The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats. / Rizqhilmi, Muhammad Faris; Hermanadi, Muhammad Ikrar; Yusuf, Muhammad et al.
6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. ed. / Siti Fauziyah Rahman; Ahmad Zakiyuddin; Yudan Whulanza; Nurul Intan. American Institute of Physics Inc., 2022. 040008 (AIP Conference Proceedings; Vol. 2537).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats

AU - Rizqhilmi, Muhammad Faris

AU - Hermanadi, Muhammad Ikrar

AU - Yusuf, Muhammad

AU - Hakim, Rani Wardani

AU - Farida, Siti

AU - Purwaningsih, Erni Hernawati

N1 - Funding Information: The researcher would like to thank the Health Research and Development Agency, Ministry of Health of the Republic of Indonesia, the Department of Pharmacy, and the Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, as well as all parties involved in this study. Publisher Copyright: © 2022 American Institute of Physics Inc.. All rights reserved.

PY - 2022/8/16

Y1 - 2022/8/16

N2 - The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.

AB - The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.

KW - aging

KW - Centella asiatica

KW - heart

KW - interleukin-10

UR - https://www.scopus.com/pages/publications/85138252652

U2 - 10.1063/5.0100545

DO - 10.1063/5.0100545

M3 - Conference contribution

AN - SCOPUS:85138252652

T3 - AIP Conference Proceedings

BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices

A2 - Rahman, Siti Fauziyah

A2 - Zakiyuddin, Ahmad

A2 - Whulanza, Yudan

A2 - Intan, Nurul

PB - American Institute of Physics Inc.

T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021

Y2 - 7 July 2021 through 8 July 2021

ER -

Rizqhilmi MF, Hermanadi MI, Yusuf M, Hakim RW , Farida S, Purwaningsih EH. The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats. In Rahman SF, Zakiyuddin A, Whulanza Y, Intan N, editors, 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. American Institute of Physics Inc. 2022. 040008. (AIP Conference Proceedings). doi: 10.1063/5.0100545

The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats

Abstract

Publication series

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UN SDGs

Keywords

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