TY - GEN
T1 - The Effect of Centella asiatica Ethanolic Extract on Heart Interleukin-10 Levels of Aged Sprague-Dawley Rats
AU - Rizqhilmi, Muhammad Faris
AU - Hermanadi, Muhammad Ikrar
AU - Yusuf, Muhammad
AU - Hakim, Rani Wardani
AU - Farida, Siti
AU - Purwaningsih, Erni Hernawati
N1 - Funding Information:
The researcher would like to thank the Health Research and Development Agency, Ministry of Health of the Republic of Indonesia, the Department of Pharmacy, and the Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, as well as all parties involved in this study.
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.
AB - The rising trend of average life expectancy increased the number of elderly populations. Aging could increase the risk of age-related diseases, such as cardiovascular diseases, through chronic inflammatory process. To inhibit aging, the consumption of herbal plants with anti-inflammatory activity such as Centella asiatica (CA) is preferred. This preliminary study aims to elucidate the effect of CA on heart interleukin-10 (IL-10) levels of aged Sprague-Dawley (SD) rats. Subjects were male SD rats, with aged rats divided into three groups: negative control (placebo), treatment (CA ethanolic extract of 300 mg/kg of body weight), and positive control (6 IU vitamin E); while young rats assigned to comparison control. After 28 days of treatment, all rats were terminated before the concentration of heart IL-10 was measured by ELISA. The obtained data were then analyzed using the Saphiro-Wilk test and one-way ANOVA test. Our result shows CA administration increased heart IL-10 concentration (16.33 ± 2.71 pg/mg in treatment group vs 10.81 ± 0.75 pg/mg in negative control), albeit statistically insignificant. In conclusion, CA ethanolic extract of 300 mg/kg of body weight for 28 days does not affect heart IL-10 levels in aged SD rats.
KW - aging
KW - Centella asiatica
KW - heart
KW - interleukin-10
UR - http://www.scopus.com/inward/record.url?scp=85138252652&partnerID=8YFLogxK
U2 - 10.1063/5.0100545
DO - 10.1063/5.0100545
M3 - Conference contribution
AN - SCOPUS:85138252652
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -